Frequently asked questions about in vivo chlorophyll fluorescence: Practical issues

Hazem M. Kalaji; Gert Schansker; Richard J. Ladle; Vasilij Goltsev; Karolina Bosa; Suleyman I. Allakhverdiev; Marian Brestic; Filippo Bussotti; Angeles Calatayud; Piotr Dąbrowski; Nabil I. Elsheery; Lorenzo Ferroni; Lucia Guidi; Sander W. Hogewoning; Anjana Jajoo; Amarendra N. Misra; Sergio G. Nebauer; Simonetta Pancaldi; Consuelo Penella; Dorothybelle Poli; Martina Pollastrini; Zdzislawa B. Romanowska-Duda; Beata Rutkowska; João Serôdio; Kancherla Suresh; Wiesław Szulc; Eduardo Tambussi; Marcos Yanniccari; Marek Zivcak

doi:10.1007/s11120-014-0024-6

Frequently asked questions about in vivo chlorophyll fluorescence: Practical issues

Hazem M. Kalaji, Gert Schansker, Richard J. Ladle, Vasilij Goltsev, Karolina Bosa, Suleyman I. Allakhverdiev, Marian Brestic, Filippo Bussotti, Angeles Calatayud, Piotr Dąbrowski, Nabil I. Elsheery, Lorenzo Ferroni, Lucia Guidi, Sander W. Hogewoning, Anjana Jajoo, Amarendra N. Misra, Sergio G. Nebauer, Simonetta Pancaldi, Consuelo Penella, Dorothybelle PoliMartina Pollastrini, Zdzislawa B. Romanowska-Duda, Beata Rutkowska, João Serôdio, Kancherla Suresh, Wiesław Szulc, Eduardo Tambussi, Marcos Yanniccari, Marek Zivcak

Research Institute for Interdisciplinary Science

Research output: Contribution to journal › Review article › peer-review

382 Citations (Scopus)

Abstract

The aim of this educational review is to provide practical information on the hardware, methodology, and the hands on application of chlorophyll (Chl) a fluorescence technology. We present the paper in a question and answer format like frequently asked questions. Although nearly all information on the application of Chl a fluorescence can be found in the literature, it is not always easily accessible. This paper is primarily aimed at scientists who have some experience with the application of Chl a fluorescence but are still in the process of discovering what it all means and how it can be used. Topics discussed are (among other things) the kind of information that can be obtained using different fluorescence techniques, the interpretation of Chl a fluorescence signals, specific applications of these techniques, and practical advice on different subjects, such as on the length of dark adaptation before measurement of the Chl a fluorescence transient. The paper also provides the physiological background for some of the applied procedures. It also serves as a source of reference for experienced scientists.

Original language	English
Pages (from-to)	121-158
Number of pages	38
Journal	Photosynthesis research
Volume	122
Issue number	2
DOIs	https://doi.org/10.1007/s11120-014-0024-6
Publication status	Published - Nov 2014

Keywords

Chlorophyll a fluorescence
Complementary techniques
Fluorescence imaging
Frequently asked questions
Photosynthesis
Plant stress monitoring

ASJC Scopus subject areas

Biochemistry
Plant Science
Cell Biology

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10.1007/s11120-014-0024-6

Cite this

Kalaji, H. M., Schansker, G., Ladle, R. J., Goltsev, V., Bosa, K., Allakhverdiev, S. I., Brestic, M., Bussotti, F., Calatayud, A., Dąbrowski, P., Elsheery, N. I., Ferroni, L., Guidi, L., Hogewoning, S. W., Jajoo, A., Misra, A. N., Nebauer, S. G., Pancaldi, S., Penella, C., ... Zivcak, M. (2014). Frequently asked questions about in vivo chlorophyll fluorescence: Practical issues. Photosynthesis research, 122(2), 121-158. https://doi.org/10.1007/s11120-014-0024-6

Frequently asked questions about in vivo chlorophyll fluorescence : Practical issues. / Kalaji, Hazem M.; Schansker, Gert; Ladle, Richard J.; Goltsev, Vasilij; Bosa, Karolina; Allakhverdiev, Suleyman I.; Brestic, Marian; Bussotti, Filippo; Calatayud, Angeles; Dąbrowski, Piotr; Elsheery, Nabil I.; Ferroni, Lorenzo; Guidi, Lucia; Hogewoning, Sander W.; Jajoo, Anjana; Misra, Amarendra N.; Nebauer, Sergio G.; Pancaldi, Simonetta; Penella, Consuelo; Poli, Dorothybelle; Pollastrini, Martina; Romanowska-Duda, Zdzislawa B.; Rutkowska, Beata; Serôdio, João; Suresh, Kancherla; Szulc, Wiesław; Tambussi, Eduardo; Yanniccari, Marcos; Zivcak, Marek.

In: Photosynthesis research, Vol. 122, No. 2, 11.2014, p. 121-158.

Research output: Contribution to journal › Review article › peer-review

Kalaji, HM, Schansker, G, Ladle, RJ, Goltsev, V, Bosa, K, Allakhverdiev, SI, Brestic, M, Bussotti, F, Calatayud, A, Dąbrowski, P, Elsheery, NI, Ferroni, L, Guidi, L, Hogewoning, SW, Jajoo, A, Misra, AN, Nebauer, SG, Pancaldi, S, Penella, C, Poli, D, Pollastrini, M, Romanowska-Duda, ZB, Rutkowska, B, Serôdio, J, Suresh, K, Szulc, W, Tambussi, E, Yanniccari, M & Zivcak, M 2014, 'Frequently asked questions about in vivo chlorophyll fluorescence: Practical issues', Photosynthesis research, vol. 122, no. 2, pp. 121-158. https://doi.org/10.1007/s11120-014-0024-6

Kalaji, Hazem M. ; Schansker, Gert ; Ladle, Richard J. ; Goltsev, Vasilij ; Bosa, Karolina ; Allakhverdiev, Suleyman I. ; Brestic, Marian ; Bussotti, Filippo ; Calatayud, Angeles ; Dąbrowski, Piotr ; Elsheery, Nabil I. ; Ferroni, Lorenzo ; Guidi, Lucia ; Hogewoning, Sander W. ; Jajoo, Anjana ; Misra, Amarendra N. ; Nebauer, Sergio G. ; Pancaldi, Simonetta ; Penella, Consuelo ; Poli, Dorothybelle ; Pollastrini, Martina ; Romanowska-Duda, Zdzislawa B. ; Rutkowska, Beata ; Serôdio, João ; Suresh, Kancherla ; Szulc, Wiesław ; Tambussi, Eduardo ; Yanniccari, Marcos ; Zivcak, Marek. / Frequently asked questions about in vivo chlorophyll fluorescence : Practical issues. In: Photosynthesis research. 2014 ; Vol. 122, No. 2. pp. 121-158.

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abstract = "The aim of this educational review is to provide practical information on the hardware, methodology, and the hands on application of chlorophyll (Chl) a fluorescence technology. We present the paper in a question and answer format like frequently asked questions. Although nearly all information on the application of Chl a fluorescence can be found in the literature, it is not always easily accessible. This paper is primarily aimed at scientists who have some experience with the application of Chl a fluorescence but are still in the process of discovering what it all means and how it can be used. Topics discussed are (among other things) the kind of information that can be obtained using different fluorescence techniques, the interpretation of Chl a fluorescence signals, specific applications of these techniques, and practical advice on different subjects, such as on the length of dark adaptation before measurement of the Chl a fluorescence transient. The paper also provides the physiological background for some of the applied procedures. It also serves as a source of reference for experienced scientists.",

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AU - Kalaji, Hazem M.

AU - Schansker, Gert

AU - Ladle, Richard J.

AU - Goltsev, Vasilij

AU - Bosa, Karolina

AU - Allakhverdiev, Suleyman I.

AU - Brestic, Marian

AU - Bussotti, Filippo

AU - Calatayud, Angeles

AU - Dąbrowski, Piotr

AU - Elsheery, Nabil I.

AU - Ferroni, Lorenzo

AU - Guidi, Lucia

AU - Hogewoning, Sander W.

AU - Jajoo, Anjana

AU - Misra, Amarendra N.

AU - Nebauer, Sergio G.

AU - Pancaldi, Simonetta

AU - Penella, Consuelo

AU - Poli, Dorothybelle

AU - Pollastrini, Martina

AU - Romanowska-Duda, Zdzislawa B.

AU - Rutkowska, Beata

AU - Serôdio, João

AU - Suresh, Kancherla

AU - Szulc, Wiesław

AU - Tambussi, Eduardo

AU - Yanniccari, Marcos

AU - Zivcak, Marek

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AB - The aim of this educational review is to provide practical information on the hardware, methodology, and the hands on application of chlorophyll (Chl) a fluorescence technology. We present the paper in a question and answer format like frequently asked questions. Although nearly all information on the application of Chl a fluorescence can be found in the literature, it is not always easily accessible. This paper is primarily aimed at scientists who have some experience with the application of Chl a fluorescence but are still in the process of discovering what it all means and how it can be used. Topics discussed are (among other things) the kind of information that can be obtained using different fluorescence techniques, the interpretation of Chl a fluorescence signals, specific applications of these techniques, and practical advice on different subjects, such as on the length of dark adaptation before measurement of the Chl a fluorescence transient. The paper also provides the physiological background for some of the applied procedures. It also serves as a source of reference for experienced scientists.

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Frequently asked questions about in vivo chlorophyll fluorescence: Practical issues

Abstract

Keywords

ASJC Scopus subject areas

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