TY - JOUR
T1 - Frequently asked questions about in vivo chlorophyll fluorescence
T2 - Practical issues
AU - Kalaji, Hazem M.
AU - Schansker, Gert
AU - Ladle, Richard J.
AU - Goltsev, Vasilij
AU - Bosa, Karolina
AU - Allakhverdiev, Suleyman I.
AU - Brestic, Marian
AU - Bussotti, Filippo
AU - Calatayud, Angeles
AU - Dąbrowski, Piotr
AU - Elsheery, Nabil I.
AU - Ferroni, Lorenzo
AU - Guidi, Lucia
AU - Hogewoning, Sander W.
AU - Jajoo, Anjana
AU - Misra, Amarendra N.
AU - Nebauer, Sergio G.
AU - Pancaldi, Simonetta
AU - Penella, Consuelo
AU - Poli, Dorothybelle
AU - Pollastrini, Martina
AU - Romanowska-Duda, Zdzislawa B.
AU - Rutkowska, Beata
AU - Serôdio, João
AU - Suresh, Kancherla
AU - Szulc, Wiesław
AU - Tambussi, Eduardo
AU - Yanniccari, Marcos
AU - Zivcak, Marek
N1 - Publisher Copyright:
© 2014 The Author(s).
PY - 2014/11
Y1 - 2014/11
N2 - The aim of this educational review is to provide practical information on the hardware, methodology, and the hands on application of chlorophyll (Chl) a fluorescence technology. We present the paper in a question and answer format like frequently asked questions. Although nearly all information on the application of Chl a fluorescence can be found in the literature, it is not always easily accessible. This paper is primarily aimed at scientists who have some experience with the application of Chl a fluorescence but are still in the process of discovering what it all means and how it can be used. Topics discussed are (among other things) the kind of information that can be obtained using different fluorescence techniques, the interpretation of Chl a fluorescence signals, specific applications of these techniques, and practical advice on different subjects, such as on the length of dark adaptation before measurement of the Chl a fluorescence transient. The paper also provides the physiological background for some of the applied procedures. It also serves as a source of reference for experienced scientists.
AB - The aim of this educational review is to provide practical information on the hardware, methodology, and the hands on application of chlorophyll (Chl) a fluorescence technology. We present the paper in a question and answer format like frequently asked questions. Although nearly all information on the application of Chl a fluorescence can be found in the literature, it is not always easily accessible. This paper is primarily aimed at scientists who have some experience with the application of Chl a fluorescence but are still in the process of discovering what it all means and how it can be used. Topics discussed are (among other things) the kind of information that can be obtained using different fluorescence techniques, the interpretation of Chl a fluorescence signals, specific applications of these techniques, and practical advice on different subjects, such as on the length of dark adaptation before measurement of the Chl a fluorescence transient. The paper also provides the physiological background for some of the applied procedures. It also serves as a source of reference for experienced scientists.
KW - Chlorophyll a fluorescence
KW - Complementary techniques
KW - Fluorescence imaging
KW - Frequently asked questions
KW - Photosynthesis
KW - Plant stress monitoring
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U2 - 10.1007/s11120-014-0024-6
DO - 10.1007/s11120-014-0024-6
M3 - Review article
C2 - 25119687
AN - SCOPUS:84926113875
VL - 122
SP - 121
EP - 158
JO - Photosynthesis Research
JF - Photosynthesis Research
SN - 0166-8595
IS - 2
ER -