Frequently asked questions about in vivo chlorophyll fluorescence: Practical issues

Hazem M. Kalaji; Gert Schansker; Richard J. Ladle; Vasilij Goltsev; Karolina Bosa; Suleyman I. Allakhverdiev; Marian Brestic; Filippo Bussotti; Angeles Calatayud; Piotr Dąbrowski; Nabil I. Elsheery; Lorenzo Ferroni; Lucia Guidi; Sander W. Hogewoning; Anjana Jajoo; Amarendra N. Misra; Sergio G. Nebauer; Simonetta Pancaldi; Consuelo Penella; Dorothybelle Poli; Martina Pollastrini; Zdzislawa B. Romanowska-Duda; Beata Rutkowska; João Serôdio; Kancherla Suresh; Wiesław Szulc; Eduardo Tambussi; Marcos Yanniccari; Marek Zivcak

doi:10.1007/s11120-014-0024-6

Frequently asked questions about in vivo chlorophyll fluorescence: Practical issues

Hazem M. Kalaji, Gert Schansker, Richard J. Ladle, Vasilij Goltsev, Karolina Bosa, Suleyman I. Allakhverdiev, Marian Brestic, Filippo Bussotti, Angeles Calatayud, Piotr Dąbrowski, Nabil I. Elsheery, Lorenzo Ferroni, Lucia Guidi, Sander W. Hogewoning, Anjana Jajoo, Amarendra N. Misra, Sergio G. Nebauer, Simonetta Pancaldi, Consuelo Penella, Dorothybelle PoliMartina Pollastrini, Zdzislawa B. Romanowska-Duda, Beata Rutkowska, João Serôdio, Kancherla Suresh, Wiesław Szulc, Eduardo Tambussi, Marcos Yanniccari, Marek Zivcak

Research output: Contribution to journal › Review article › peer-review

509 Citations (Scopus)

Abstract

The aim of this educational review is to provide practical information on the hardware, methodology, and the hands on application of chlorophyll (Chl) a fluorescence technology. We present the paper in a question and answer format like frequently asked questions. Although nearly all information on the application of Chl a fluorescence can be found in the literature, it is not always easily accessible. This paper is primarily aimed at scientists who have some experience with the application of Chl a fluorescence but are still in the process of discovering what it all means and how it can be used. Topics discussed are (among other things) the kind of information that can be obtained using different fluorescence techniques, the interpretation of Chl a fluorescence signals, specific applications of these techniques, and practical advice on different subjects, such as on the length of dark adaptation before measurement of the Chl a fluorescence transient. The paper also provides the physiological background for some of the applied procedures. It also serves as a source of reference for experienced scientists.

Original language	English
Pages (from-to)	121-158
Number of pages	38
Journal	Photosynthesis research
Volume	122
Issue number	2
DOIs	https://doi.org/10.1007/s11120-014-0024-6
Publication status	Published - Nov 2014
Externally published	Yes

Keywords

Chlorophyll a fluorescence
Complementary techniques
Fluorescence imaging
Frequently asked questions
Photosynthesis
Plant stress monitoring

ASJC Scopus subject areas

Biochemistry
Plant Science
Cell Biology

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10.1007/s11120-014-0024-6

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Kalaji, H. M., Schansker, G., Ladle, R. J., Goltsev, V., Bosa, K., Allakhverdiev, S. I., Brestic, M., Bussotti, F., Calatayud, A., Dąbrowski, P., Elsheery, N. I., Ferroni, L., Guidi, L., Hogewoning, S. W., Jajoo, A., Misra, A. N., Nebauer, S. G., Pancaldi, S., Penella, C., ... Zivcak, M. (2014). Frequently asked questions about in vivo chlorophyll fluorescence: Practical issues. Photosynthesis research, 122(2), 121-158. https://doi.org/10.1007/s11120-014-0024-6

Kalaji, HM, Schansker, G, Ladle, RJ, Goltsev, V, Bosa, K, Allakhverdiev, SI, Brestic, M, Bussotti, F, Calatayud, A, Dąbrowski, P, Elsheery, NI, Ferroni, L, Guidi, L, Hogewoning, SW, Jajoo, A, Misra, AN, Nebauer, SG, Pancaldi, S, Penella, C, Poli, D, Pollastrini, M, Romanowska-Duda, ZB, Rutkowska, B, Serôdio, J, Suresh, K, Szulc, W, Tambussi, E, Yanniccari, M & Zivcak, M 2014, 'Frequently asked questions about in vivo chlorophyll fluorescence: Practical issues', Photosynthesis research, vol. 122, no. 2, pp. 121-158. https://doi.org/10.1007/s11120-014-0024-6

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title = "Frequently asked questions about in vivo chlorophyll fluorescence: Practical issues",

abstract = "The aim of this educational review is to provide practical information on the hardware, methodology, and the hands on application of chlorophyll (Chl) a fluorescence technology. We present the paper in a question and answer format like frequently asked questions. Although nearly all information on the application of Chl a fluorescence can be found in the literature, it is not always easily accessible. This paper is primarily aimed at scientists who have some experience with the application of Chl a fluorescence but are still in the process of discovering what it all means and how it can be used. Topics discussed are (among other things) the kind of information that can be obtained using different fluorescence techniques, the interpretation of Chl a fluorescence signals, specific applications of these techniques, and practical advice on different subjects, such as on the length of dark adaptation before measurement of the Chl a fluorescence transient. The paper also provides the physiological background for some of the applied procedures. It also serves as a source of reference for experienced scientists.",

keywords = "Chlorophyll a fluorescence, Complementary techniques, Fluorescence imaging, Frequently asked questions, Photosynthesis, Plant stress monitoring",

author = "Kalaji, {Hazem M.} and Gert Schansker and Ladle, {Richard J.} and Vasilij Goltsev and Karolina Bosa and Allakhverdiev, {Suleyman I.} and Marian Brestic and Filippo Bussotti and Angeles Calatayud and Piotr D{\c a}browski and Elsheery, {Nabil I.} and Lorenzo Ferroni and Lucia Guidi and Hogewoning, {Sander W.} and Anjana Jajoo and Misra, {Amarendra N.} and Nebauer, {Sergio G.} and Simonetta Pancaldi and Consuelo Penella and Dorothybelle Poli and Martina Pollastrini and Romanowska-Duda, {Zdzislawa B.} and Beata Rutkowska and Jo{\~a}o Ser{\^o}dio and Kancherla Suresh and Wies{\l}aw Szulc and Eduardo Tambussi and Marcos Yanniccari and Marek Zivcak",

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year = "2014",

month = nov,

doi = "10.1007/s11120-014-0024-6",

language = "English",

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T1 - Frequently asked questions about in vivo chlorophyll fluorescence

T2 - Practical issues

AU - Kalaji, Hazem M.

AU - Schansker, Gert

AU - Ladle, Richard J.

AU - Goltsev, Vasilij

AU - Bosa, Karolina

AU - Allakhverdiev, Suleyman I.

AU - Brestic, Marian

AU - Bussotti, Filippo

AU - Calatayud, Angeles

AU - Dąbrowski, Piotr

AU - Elsheery, Nabil I.

AU - Ferroni, Lorenzo

AU - Guidi, Lucia

AU - Hogewoning, Sander W.

AU - Jajoo, Anjana

AU - Misra, Amarendra N.

AU - Nebauer, Sergio G.

AU - Pancaldi, Simonetta

AU - Penella, Consuelo

AU - Poli, Dorothybelle

AU - Pollastrini, Martina

AU - Romanowska-Duda, Zdzislawa B.

AU - Rutkowska, Beata

AU - Serôdio, João

AU - Suresh, Kancherla

AU - Szulc, Wiesław

AU - Tambussi, Eduardo

AU - Yanniccari, Marcos

AU - Zivcak, Marek

PY - 2014/11

Y1 - 2014/11

N2 - The aim of this educational review is to provide practical information on the hardware, methodology, and the hands on application of chlorophyll (Chl) a fluorescence technology. We present the paper in a question and answer format like frequently asked questions. Although nearly all information on the application of Chl a fluorescence can be found in the literature, it is not always easily accessible. This paper is primarily aimed at scientists who have some experience with the application of Chl a fluorescence but are still in the process of discovering what it all means and how it can be used. Topics discussed are (among other things) the kind of information that can be obtained using different fluorescence techniques, the interpretation of Chl a fluorescence signals, specific applications of these techniques, and practical advice on different subjects, such as on the length of dark adaptation before measurement of the Chl a fluorescence transient. The paper also provides the physiological background for some of the applied procedures. It also serves as a source of reference for experienced scientists.

AB - The aim of this educational review is to provide practical information on the hardware, methodology, and the hands on application of chlorophyll (Chl) a fluorescence technology. We present the paper in a question and answer format like frequently asked questions. Although nearly all information on the application of Chl a fluorescence can be found in the literature, it is not always easily accessible. This paper is primarily aimed at scientists who have some experience with the application of Chl a fluorescence but are still in the process of discovering what it all means and how it can be used. Topics discussed are (among other things) the kind of information that can be obtained using different fluorescence techniques, the interpretation of Chl a fluorescence signals, specific applications of these techniques, and practical advice on different subjects, such as on the length of dark adaptation before measurement of the Chl a fluorescence transient. The paper also provides the physiological background for some of the applied procedures. It also serves as a source of reference for experienced scientists.

KW - Chlorophyll a fluorescence

KW - Complementary techniques

KW - Fluorescence imaging

KW - Frequently asked questions

KW - Photosynthesis

KW - Plant stress monitoring

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JO - Photosynthesis Research

JF - Photosynthesis Research

SN - 0166-8595

IS - 2

ER -

Frequently asked questions about in vivo chlorophyll fluorescence: Practical issues

Abstract

Keywords

ASJC Scopus subject areas

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