Frequent silencing of protocadherin 17, a candidate tumour suppressor for esophageal squamous cell carcinoma

Shigeo Haruki, Issei Imoto, Ken Ichi Kozaki, Takeshi Matsui, Hiroshi Kawachi, Shuhei Komatsu, Tomoki Muramatsu, Yutaka Shimada, Tatsuyuki Kawano, Johji Inazawa

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Protocadherins are a subfamily of the cadherin superfamily, but little is known about their functions. We identified a homozygous loss of protocadherin (PCDH) 17 in the course of a program to screen a panel of esophageal squamous cell carcinoma (ESCC) cell lines for genomic copy number aberrations. PCDH17 messenger RNAwas expressed in normal esophageal tissue but not in the majority of ESCC cell lines without a homozygous deletion of this gene and restored in gene-silenced ESCC cells after treatment with 5-aza-2'-deoxycytidine. The DNA methylation status of the PCDH17 CpG island correlated inversely with the PCDH17 expression, and a putative methylation target region showed promoter activity. The methylation of the PCDH17 promoter was also associated with the silencing of gene expression in primary ESCC partly. Among primary ESCC cases, the silencing of PCDH17 protein expression was associated with a poorer differentiation status of ESCC cells and possibly with prognosis in a subset of this tumour. Restoration of PCDH17 expression in ESCC cells reduced cell proliferation and migration/invasion. These results suggest that silencing of PCDH17 expression through hypermethylation of the promoter or other mechanisms leads to loss of its tumour-suppressive activity, which may be a factor in the carcinogenesis of a subgroup of ESCCs.

Original languageEnglish
Pages (from-to)1027-1036
Number of pages10
JournalCarcinogenesis
Volume31
Issue number6
DOIs
Publication statusPublished - Mar 3 2010
Externally publishedYes

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Neoplasms
decitabine
Methylation
Cell Line
CpG Islands
Gene Deletion
DNA Methylation
Cadherins
Esophageal Squamous Cell Carcinoma
Genetic Promoter Regions
Cell Movement
Carcinogenesis
Cell Proliferation
Gene Expression
Genes
Proteins

ASJC Scopus subject areas

  • Cancer Research

Cite this

Haruki, S., Imoto, I., Kozaki, K. I., Matsui, T., Kawachi, H., Komatsu, S., ... Inazawa, J. (2010). Frequent silencing of protocadherin 17, a candidate tumour suppressor for esophageal squamous cell carcinoma. Carcinogenesis, 31(6), 1027-1036. https://doi.org/10.1093/carcin/bgq053

Frequent silencing of protocadherin 17, a candidate tumour suppressor for esophageal squamous cell carcinoma. / Haruki, Shigeo; Imoto, Issei; Kozaki, Ken Ichi; Matsui, Takeshi; Kawachi, Hiroshi; Komatsu, Shuhei; Muramatsu, Tomoki; Shimada, Yutaka; Kawano, Tatsuyuki; Inazawa, Johji.

In: Carcinogenesis, Vol. 31, No. 6, 03.03.2010, p. 1027-1036.

Research output: Contribution to journalArticle

Haruki, S, Imoto, I, Kozaki, KI, Matsui, T, Kawachi, H, Komatsu, S, Muramatsu, T, Shimada, Y, Kawano, T & Inazawa, J 2010, 'Frequent silencing of protocadherin 17, a candidate tumour suppressor for esophageal squamous cell carcinoma', Carcinogenesis, vol. 31, no. 6, pp. 1027-1036. https://doi.org/10.1093/carcin/bgq053
Haruki, Shigeo ; Imoto, Issei ; Kozaki, Ken Ichi ; Matsui, Takeshi ; Kawachi, Hiroshi ; Komatsu, Shuhei ; Muramatsu, Tomoki ; Shimada, Yutaka ; Kawano, Tatsuyuki ; Inazawa, Johji. / Frequent silencing of protocadherin 17, a candidate tumour suppressor for esophageal squamous cell carcinoma. In: Carcinogenesis. 2010 ; Vol. 31, No. 6. pp. 1027-1036.
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