Frequent methylation and oncogenic role of microRNA-34b/c in small-cell lung cancer

Norimitsu Tanaka, Shinichi Toyooka, Junichi Sou, Takafumi Kubo, Hiromasa Yamamoto, Yuho Maki, Takayuki Muraoka, Kazuhiko Shien, Masashi Furukawa, Tsuyoshi Ueno, Hiroaki Asano, Kazunori Tsukuda, Keisuke Aoe, Shinichiro Miyoshi

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78 Citations (Scopus)

Abstract

Small-cell lung cancer (SCLC) is an aggressive tumor with a dismal prognosis among primary lung cancers. MicroRNAs (miRNAs) can act as oncogenes or tumor-suppressor genes in human malignancy. The miR-34 family is comprised of tumor-suppressive miRNAs, and its reduced expression by methylation has been reported in various cancers, including non-small cell lung cancer (NSCLC). In this study, we investigated the alteration and tumor-suppressive impact of miR-34s in SCLC. The methylation of miR-34a and miR-34b/c was observed in 4 (36%) and 7 (64%) of 11 SCLC cell lines, respectively. Among the 27 SCLC clinical specimens, miR-34a and miR-34b/c were methylated in 4 (15%) and 18 (67%), respectively. In contrast, 13 (28%) miR-34a methylated cases and 12 (26%) miR-34b/c methylated cases were found in 47 NSCLC primary tumors. The frequency of miR-34b/c methylation was significantly higher in SCLC than in NSCLC (p

Original languageEnglish
Pages (from-to)32-38
Number of pages7
JournalLung Cancer
Volume76
Issue number1
DOIs
Publication statusPublished - Apr 2012

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Keywords

  • Methylation
  • MicroRNA
  • MicroRNA-34b/c
  • Non-small cell lung cancer
  • P53
  • Small cell lung cancer

ASJC Scopus subject areas

  • Oncology
  • Pulmonary and Respiratory Medicine
  • Cancer Research

Cite this

Tanaka, N., Toyooka, S., Sou, J., Kubo, T., Yamamoto, H., Maki, Y., Muraoka, T., Shien, K., Furukawa, M., Ueno, T., Asano, H., Tsukuda, K., Aoe, K., & Miyoshi, S. (2012). Frequent methylation and oncogenic role of microRNA-34b/c in small-cell lung cancer. Lung Cancer, 76(1), 32-38. https://doi.org/10.1016/j.lungcan.2011.10.002