Frequency of regulatory T-cell and hepatitis C viral antigen-specific immune response in recurrent hepatitis C after liver transplantation

Masashi Utsumi, Akinobu Takaki, Yuzo Umeda, Kazuko Koike, Stephanie C. Napier, Nobukazu Watanabe, Hiroshi Sadamori, Susumu Shinoura, Ryuichi Yoshida, Daisuke Nobuoka, Tetsuya Yasunaka, Eiichi Nakayama, Kazuhide Yamamoto, Toshiyoshi Fujiwara, Takahito Yagi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Introduction: Regulatory T (Treg) and type 1 regulatory T (Tr1) cells facilitate hepatitis C virus (HCV) recurrence after orthotopic liver transplantation (OLT). However, their frequencies and effects on HCV-specific immune responses have not been well investigated. Methods: We determined Treg and Tr1 frequencies in OLT patients with hepatitis C and assessed their associations with HCV-specific T cell responses. These patients comprised the following groups: an early post-transplantation group (n=14); an OLT-chronic active hepatitis C group (n=14) with active hepatitis C (alanine aminotransferase of. >. upper limit of normal/positive for HCV-RNA); an OLT-persistently normal alanine aminotransferase group (n=12) without active hepatitis C (not interferon/positive for HCV-RNA); and an OLT-sustained viral response group (n=6) with sustained viral responses using interferon treatment (negative for HCV-RNA). The frequencies of HCV-specific CD4. + T cells that secreted interferon-γ were determined by enzyme-linked immunosorbent spot assay (except for the OLT early group). Results: Treg and Tr1 frequencies were low during the early post-transplantation period. OLT patients with sustained viral responses had lower Treg frequencies than those with chronic hepatitis C, whereas Tr1 frequencies were significantly reduced in OLT patients with persistently normal alanine aminotransferase levels compared to those with chronic hepatitis C (p.

Original languageEnglish
Pages (from-to)33-41
Number of pages9
JournalTransplant Immunology
Volume31
Issue number1
DOIs
Publication statusPublished - 2014

Fingerprint

Hepatitis C Antigens
Viral Antigens
Regulatory T-Lymphocytes
Hepatitis C
Liver Transplantation
Hepacivirus
Chronic Hepatitis C
Alanine Transaminase
Interferons
RNA
Transplantation
T-Lymphocytes
Chronic Hepatitis
Enzyme-Linked Immunosorbent Assay
Recurrence

Keywords

  • Chronic hepatitis C
  • Living donor liver transplantation
  • Persistently normal alanine aminotransferase
  • Regulatory T cell
  • Sustained viral response
  • Type 1 regulatory T cells

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Transplantation
  • Medicine(all)

Cite this

Frequency of regulatory T-cell and hepatitis C viral antigen-specific immune response in recurrent hepatitis C after liver transplantation. / Utsumi, Masashi; Takaki, Akinobu; Umeda, Yuzo; Koike, Kazuko; Napier, Stephanie C.; Watanabe, Nobukazu; Sadamori, Hiroshi; Shinoura, Susumu; Yoshida, Ryuichi; Nobuoka, Daisuke; Yasunaka, Tetsuya; Nakayama, Eiichi; Yamamoto, Kazuhide; Fujiwara, Toshiyoshi; Yagi, Takahito.

In: Transplant Immunology, Vol. 31, No. 1, 2014, p. 33-41.

Research output: Contribution to journalArticle

Utsumi, Masashi ; Takaki, Akinobu ; Umeda, Yuzo ; Koike, Kazuko ; Napier, Stephanie C. ; Watanabe, Nobukazu ; Sadamori, Hiroshi ; Shinoura, Susumu ; Yoshida, Ryuichi ; Nobuoka, Daisuke ; Yasunaka, Tetsuya ; Nakayama, Eiichi ; Yamamoto, Kazuhide ; Fujiwara, Toshiyoshi ; Yagi, Takahito. / Frequency of regulatory T-cell and hepatitis C viral antigen-specific immune response in recurrent hepatitis C after liver transplantation. In: Transplant Immunology. 2014 ; Vol. 31, No. 1. pp. 33-41.
@article{abd4506b3e6c460cb073d5a504f18593,
title = "Frequency of regulatory T-cell and hepatitis C viral antigen-specific immune response in recurrent hepatitis C after liver transplantation",
abstract = "Introduction: Regulatory T (Treg) and type 1 regulatory T (Tr1) cells facilitate hepatitis C virus (HCV) recurrence after orthotopic liver transplantation (OLT). However, their frequencies and effects on HCV-specific immune responses have not been well investigated. Methods: We determined Treg and Tr1 frequencies in OLT patients with hepatitis C and assessed their associations with HCV-specific T cell responses. These patients comprised the following groups: an early post-transplantation group (n=14); an OLT-chronic active hepatitis C group (n=14) with active hepatitis C (alanine aminotransferase of. >. upper limit of normal/positive for HCV-RNA); an OLT-persistently normal alanine aminotransferase group (n=12) without active hepatitis C (not interferon/positive for HCV-RNA); and an OLT-sustained viral response group (n=6) with sustained viral responses using interferon treatment (negative for HCV-RNA). The frequencies of HCV-specific CD4. + T cells that secreted interferon-γ were determined by enzyme-linked immunosorbent spot assay (except for the OLT early group). Results: Treg and Tr1 frequencies were low during the early post-transplantation period. OLT patients with sustained viral responses had lower Treg frequencies than those with chronic hepatitis C, whereas Tr1 frequencies were significantly reduced in OLT patients with persistently normal alanine aminotransferase levels compared to those with chronic hepatitis C (p.",
keywords = "Chronic hepatitis C, Living donor liver transplantation, Persistently normal alanine aminotransferase, Regulatory T cell, Sustained viral response, Type 1 regulatory T cells",
author = "Masashi Utsumi and Akinobu Takaki and Yuzo Umeda and Kazuko Koike and Napier, {Stephanie C.} and Nobukazu Watanabe and Hiroshi Sadamori and Susumu Shinoura and Ryuichi Yoshida and Daisuke Nobuoka and Tetsuya Yasunaka and Eiichi Nakayama and Kazuhide Yamamoto and Toshiyoshi Fujiwara and Takahito Yagi",
year = "2014",
doi = "10.1016/j.trim.2014.05.006",
language = "English",
volume = "31",
pages = "33--41",
journal = "Transplant Immunology",
issn = "0966-3274",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Frequency of regulatory T-cell and hepatitis C viral antigen-specific immune response in recurrent hepatitis C after liver transplantation

AU - Utsumi, Masashi

AU - Takaki, Akinobu

AU - Umeda, Yuzo

AU - Koike, Kazuko

AU - Napier, Stephanie C.

AU - Watanabe, Nobukazu

AU - Sadamori, Hiroshi

AU - Shinoura, Susumu

AU - Yoshida, Ryuichi

AU - Nobuoka, Daisuke

AU - Yasunaka, Tetsuya

AU - Nakayama, Eiichi

AU - Yamamoto, Kazuhide

AU - Fujiwara, Toshiyoshi

AU - Yagi, Takahito

PY - 2014

Y1 - 2014

N2 - Introduction: Regulatory T (Treg) and type 1 regulatory T (Tr1) cells facilitate hepatitis C virus (HCV) recurrence after orthotopic liver transplantation (OLT). However, their frequencies and effects on HCV-specific immune responses have not been well investigated. Methods: We determined Treg and Tr1 frequencies in OLT patients with hepatitis C and assessed their associations with HCV-specific T cell responses. These patients comprised the following groups: an early post-transplantation group (n=14); an OLT-chronic active hepatitis C group (n=14) with active hepatitis C (alanine aminotransferase of. >. upper limit of normal/positive for HCV-RNA); an OLT-persistently normal alanine aminotransferase group (n=12) without active hepatitis C (not interferon/positive for HCV-RNA); and an OLT-sustained viral response group (n=6) with sustained viral responses using interferon treatment (negative for HCV-RNA). The frequencies of HCV-specific CD4. + T cells that secreted interferon-γ were determined by enzyme-linked immunosorbent spot assay (except for the OLT early group). Results: Treg and Tr1 frequencies were low during the early post-transplantation period. OLT patients with sustained viral responses had lower Treg frequencies than those with chronic hepatitis C, whereas Tr1 frequencies were significantly reduced in OLT patients with persistently normal alanine aminotransferase levels compared to those with chronic hepatitis C (p.

AB - Introduction: Regulatory T (Treg) and type 1 regulatory T (Tr1) cells facilitate hepatitis C virus (HCV) recurrence after orthotopic liver transplantation (OLT). However, their frequencies and effects on HCV-specific immune responses have not been well investigated. Methods: We determined Treg and Tr1 frequencies in OLT patients with hepatitis C and assessed their associations with HCV-specific T cell responses. These patients comprised the following groups: an early post-transplantation group (n=14); an OLT-chronic active hepatitis C group (n=14) with active hepatitis C (alanine aminotransferase of. >. upper limit of normal/positive for HCV-RNA); an OLT-persistently normal alanine aminotransferase group (n=12) without active hepatitis C (not interferon/positive for HCV-RNA); and an OLT-sustained viral response group (n=6) with sustained viral responses using interferon treatment (negative for HCV-RNA). The frequencies of HCV-specific CD4. + T cells that secreted interferon-γ were determined by enzyme-linked immunosorbent spot assay (except for the OLT early group). Results: Treg and Tr1 frequencies were low during the early post-transplantation period. OLT patients with sustained viral responses had lower Treg frequencies than those with chronic hepatitis C, whereas Tr1 frequencies were significantly reduced in OLT patients with persistently normal alanine aminotransferase levels compared to those with chronic hepatitis C (p.

KW - Chronic hepatitis C

KW - Living donor liver transplantation

KW - Persistently normal alanine aminotransferase

KW - Regulatory T cell

KW - Sustained viral response

KW - Type 1 regulatory T cells

UR - http://www.scopus.com/inward/record.url?scp=84903794503&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84903794503&partnerID=8YFLogxK

U2 - 10.1016/j.trim.2014.05.006

DO - 10.1016/j.trim.2014.05.006

M3 - Article

C2 - 24907425

AN - SCOPUS:84903794503

VL - 31

SP - 33

EP - 41

JO - Transplant Immunology

JF - Transplant Immunology

SN - 0966-3274

IS - 1

ER -