Free radicals and oxidative stress: Targeted ESR measurement of free radicals

Toru Egashira, Fusako Takayama

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The detection of free radicals generated within the body may contribute to clarifying the pathophysiological role of free radicals in disease processes. As an appropriate procedure to examine the generation of free radicals in a biological system, electron spin resonance (ESR) has emerged as a powerful tool for detection and identification. A method for determination of oxygen radical scavenging activity using ESR and the spin trapping technique was developed. Oxygen radicals were trapped by 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) or α-phenyl-N-t-butylnitrone (PBN), and the DMPO or PBN spin aduct signal was measured quantitatively by an ESR spectrometer. The spin trapping method using ESR has also been reported for not only in vitro and ex vivo measurements but also in vivo measurements. In in vivo ESR, nitroxyl radical is being used as a spin trap well. ESR signal intensities of nitroxyl radical are measured after administration to animals and the signal decay rates of nitroxyl radical have reported to be influenced by various types of oxidative stress. With this method, it is possible to specify the type of radical or the location at which the free radicals are produced. The spin trapping method by in vivo ESR is an effective procedure for giving non-invasive measurements in animals. ESR imaging in the organs of live animals can also be obtained after injection of nitroxyl radicals as an imaging agent using ESR-computed tomography. In vivo ESR imaging has been established as a powerful technique for determining the spatial distribution of free radicals in living organs and tissues.

Original languageEnglish
Pages (from-to)229-236
Number of pages8
JournalFolia Pharmacologica Japonica
Volume120
Issue number4
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Electron Spin Resonance Spectroscopy
Free Radicals
Oxidative Stress
Spin Trapping
Reactive Oxygen Species
Animal Structures
Oxides
Tomography
Injections
nitroxyl

Keywords

  • ESR
  • Free radical
  • In vivo ESR imaging
  • Oxidative stress
  • Spin traps

ASJC Scopus subject areas

  • Pharmacology

Cite this

Free radicals and oxidative stress : Targeted ESR measurement of free radicals. / Egashira, Toru; Takayama, Fusako.

In: Folia Pharmacologica Japonica, Vol. 120, No. 4, 2002, p. 229-236.

Research output: Contribution to journalArticle

@article{4872c2a5cb174e148ea5dc492016c0aa,
title = "Free radicals and oxidative stress: Targeted ESR measurement of free radicals",
abstract = "The detection of free radicals generated within the body may contribute to clarifying the pathophysiological role of free radicals in disease processes. As an appropriate procedure to examine the generation of free radicals in a biological system, electron spin resonance (ESR) has emerged as a powerful tool for detection and identification. A method for determination of oxygen radical scavenging activity using ESR and the spin trapping technique was developed. Oxygen radicals were trapped by 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) or α-phenyl-N-t-butylnitrone (PBN), and the DMPO or PBN spin aduct signal was measured quantitatively by an ESR spectrometer. The spin trapping method using ESR has also been reported for not only in vitro and ex vivo measurements but also in vivo measurements. In in vivo ESR, nitroxyl radical is being used as a spin trap well. ESR signal intensities of nitroxyl radical are measured after administration to animals and the signal decay rates of nitroxyl radical have reported to be influenced by various types of oxidative stress. With this method, it is possible to specify the type of radical or the location at which the free radicals are produced. The spin trapping method by in vivo ESR is an effective procedure for giving non-invasive measurements in animals. ESR imaging in the organs of live animals can also be obtained after injection of nitroxyl radicals as an imaging agent using ESR-computed tomography. In vivo ESR imaging has been established as a powerful technique for determining the spatial distribution of free radicals in living organs and tissues.",
keywords = "ESR, Free radical, In vivo ESR imaging, Oxidative stress, Spin traps",
author = "Toru Egashira and Fusako Takayama",
year = "2002",
doi = "10.1254/fpj.120.229",
language = "English",
volume = "120",
pages = "229--236",
journal = "Folia Pharmacologica Japonica",
issn = "0015-5691",
publisher = "Japanese Pharmacological Society",
number = "4",

}

TY - JOUR

T1 - Free radicals and oxidative stress

T2 - Targeted ESR measurement of free radicals

AU - Egashira, Toru

AU - Takayama, Fusako

PY - 2002

Y1 - 2002

N2 - The detection of free radicals generated within the body may contribute to clarifying the pathophysiological role of free radicals in disease processes. As an appropriate procedure to examine the generation of free radicals in a biological system, electron spin resonance (ESR) has emerged as a powerful tool for detection and identification. A method for determination of oxygen radical scavenging activity using ESR and the spin trapping technique was developed. Oxygen radicals were trapped by 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) or α-phenyl-N-t-butylnitrone (PBN), and the DMPO or PBN spin aduct signal was measured quantitatively by an ESR spectrometer. The spin trapping method using ESR has also been reported for not only in vitro and ex vivo measurements but also in vivo measurements. In in vivo ESR, nitroxyl radical is being used as a spin trap well. ESR signal intensities of nitroxyl radical are measured after administration to animals and the signal decay rates of nitroxyl radical have reported to be influenced by various types of oxidative stress. With this method, it is possible to specify the type of radical or the location at which the free radicals are produced. The spin trapping method by in vivo ESR is an effective procedure for giving non-invasive measurements in animals. ESR imaging in the organs of live animals can also be obtained after injection of nitroxyl radicals as an imaging agent using ESR-computed tomography. In vivo ESR imaging has been established as a powerful technique for determining the spatial distribution of free radicals in living organs and tissues.

AB - The detection of free radicals generated within the body may contribute to clarifying the pathophysiological role of free radicals in disease processes. As an appropriate procedure to examine the generation of free radicals in a biological system, electron spin resonance (ESR) has emerged as a powerful tool for detection and identification. A method for determination of oxygen radical scavenging activity using ESR and the spin trapping technique was developed. Oxygen radicals were trapped by 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) or α-phenyl-N-t-butylnitrone (PBN), and the DMPO or PBN spin aduct signal was measured quantitatively by an ESR spectrometer. The spin trapping method using ESR has also been reported for not only in vitro and ex vivo measurements but also in vivo measurements. In in vivo ESR, nitroxyl radical is being used as a spin trap well. ESR signal intensities of nitroxyl radical are measured after administration to animals and the signal decay rates of nitroxyl radical have reported to be influenced by various types of oxidative stress. With this method, it is possible to specify the type of radical or the location at which the free radicals are produced. The spin trapping method by in vivo ESR is an effective procedure for giving non-invasive measurements in animals. ESR imaging in the organs of live animals can also be obtained after injection of nitroxyl radicals as an imaging agent using ESR-computed tomography. In vivo ESR imaging has been established as a powerful technique for determining the spatial distribution of free radicals in living organs and tissues.

KW - ESR

KW - Free radical

KW - In vivo ESR imaging

KW - Oxidative stress

KW - Spin traps

UR - http://www.scopus.com/inward/record.url?scp=0036386785&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036386785&partnerID=8YFLogxK

U2 - 10.1254/fpj.120.229

DO - 10.1254/fpj.120.229

M3 - Article

C2 - 12425148

AN - SCOPUS:0036386785

VL - 120

SP - 229

EP - 236

JO - Folia Pharmacologica Japonica

JF - Folia Pharmacologica Japonica

SN - 0015-5691

IS - 4

ER -