Four plasmids simultaneously maintained in bacillus cereus, a tetracycline-resistant isolate

Haruhisa Kobayashi-Saegusa, Satoru Iwamori, Reiko Sakaguchi, Yoshihiro Sumita, Norihisa Noguchi, Kazuo Shishido

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Abstract

We have isolated a tetracycline-resistant (Tcr) Bacillus species (named HE-1) which carries multiple plasmids. HE-1 was identified as Bacillus cereus and found to bear four plasmids. Tetracycline resistance could be attributed to one of four plasmids (designated as pTITβ2 (4.7 kb)) indistinguishable from pBCI6, a Tcr plasmid formerly found in B. cereus [K. Bernhard, H. Schrempf, and W. Goebel, J. Bacteriol., 133, 897 (1978)]. All the other three plasmids (named pTITα (4.0kb), pTITβ1 (4.7kb) and pTITγ (12.4kb)) were cryptic and did not correlate with bacterial phenotypic traits such as antibiotic resistance or antibiotic and bacteriocin production. B. cereus HE-l alsb showed resistance to penicillin, but this seemed very likely to be chromosomally determined in B. cereus. Of interest was the fact that pTITα, pTITβ1, and pTiTγ had a noticeable DNA homology among them in blot hybridization. pTITβ2 alone did not shared sequence homology with the other three plasmids.

Original languageEnglish
Pages (from-to)1665-1670
Number of pages6
JournalAgricultural and Biological Chemistry
Volume51
Issue number6
DOIs
Publication statusPublished - Jun 1987

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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    Kobayashi-Saegusa, H., Iwamori, S., Sakaguchi, R., Sumita, Y., Noguchi, N., & Shishido, K. (1987). Four plasmids simultaneously maintained in bacillus cereus, a tetracycline-resistant isolate. Agricultural and Biological Chemistry, 51(6), 1665-1670. https://doi.org/10.1080/00021369.1987.10868247