We have isolated a tetracycline-resistant (Tcr) Bacillus species (named HE-1) which carries multiple plasmids. HE-1 was identified as Bacillus cereus and found to bear four plasmids. Tetracycline resistance could be attributed to one of four plasmids (designated as pTITB2 (4.7 kb)) indistinguishable from pBC16, a Tcr plasmid formerly found in B. cereus [K. Bernhard, H. Schrempf, and W. Goebel, J. Bacteriol., 133, 897 (1978)]. All the other three plasmids (named pTITa (4.0 kb), pTITBl (4.7 kb) and pTITy (12.4kb)) were cryptic and did not correlate with bacterial phenotypic traits such as antibiotic resistance or antibiotic and bacteriocin production. B. cereus HE-1 also showed resistance to penicillin, but this seemed very likely to be chromosomally determined in B. cereus. Of interest was the fact that pTITa, pTITBl, and pTITy had a noticeable DNA homology among them in blot hybridization. pTITB2 alone did not shared sequence homology with the other three plasmids.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Agricultural and Biological Sciences(all)