Four Plasmids Simultaneously Maintained in Bacillus cereus, a Tetracycline-resistant Isolate

Haruhisa Kobayashi-Saegusa, Satoru Iwamori, Reiko Sakaguchi, Yoshihiro Sumita, Kazuo Shishido, Norihisa Noguchi

Research output: Contribution to journalArticle

Abstract

We have isolated a tetracycline-resistant (Tcr) Bacillus species (named HE-1) which carries multiple plasmids. HE-1 was identified as Bacillus cereus and found to bear four plasmids. Tetracycline resistance could be attributed to one of four plasmids (designated as pTITB2 (4.7 kb)) indistinguishable from pBC16, a Tcr plasmid formerly found in B. cereus [K. Bernhard, H. Schrempf, and W. Goebel, J. Bacteriol., 133, 897 (1978)]. All the other three plasmids (named pTITa (4.0 kb), pTITBl (4.7 kb) and pTITy (12.4kb)) were cryptic and did not correlate with bacterial phenotypic traits such as antibiotic resistance or antibiotic and bacteriocin production. B. cereus HE-1 also showed resistance to penicillin, but this seemed very likely to be chromosomally determined in B. cereus. Of interest was the fact that pTITa, pTITBl, and pTITy had a noticeable DNA homology among them in blot hybridization. pTITB2 alone did not shared sequence homology with the other three plasmids.

Original languageEnglish
Pages (from-to)1665-1670
Number of pages6
JournalAgricultural and Biological Chemistry
Volume51
Issue number6
DOIs
Publication statusPublished - Jan 1 1987
Externally publishedYes

Fingerprint

Bacillus cereus
Tetracycline
tetracycline
plasmids
Plasmids
Anti-Bacterial Agents
Penicillin Resistance
Tetracycline Resistance
Bacteriocins
Bacillus (bacteria)
bacteriocins
Bacilli
penicillins
Microbial Drug Resistance
Sequence Homology
sequence homology
antibiotic resistance
Penicillins
Bacillus
hybridization

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Kobayashi-Saegusa, H., Iwamori, S., Sakaguchi, R., Sumita, Y., Shishido, K., & Noguchi, N. (1987). Four Plasmids Simultaneously Maintained in Bacillus cereus, a Tetracycline-resistant Isolate. Agricultural and Biological Chemistry, 51(6), 1665-1670. https://doi.org/10.1271/bbb1961.51.1665

Four Plasmids Simultaneously Maintained in Bacillus cereus, a Tetracycline-resistant Isolate. / Kobayashi-Saegusa, Haruhisa; Iwamori, Satoru; Sakaguchi, Reiko; Sumita, Yoshihiro; Shishido, Kazuo; Noguchi, Norihisa.

In: Agricultural and Biological Chemistry, Vol. 51, No. 6, 01.01.1987, p. 1665-1670.

Research output: Contribution to journalArticle

Kobayashi-Saegusa, H, Iwamori, S, Sakaguchi, R, Sumita, Y, Shishido, K & Noguchi, N 1987, 'Four Plasmids Simultaneously Maintained in Bacillus cereus, a Tetracycline-resistant Isolate', Agricultural and Biological Chemistry, vol. 51, no. 6, pp. 1665-1670. https://doi.org/10.1271/bbb1961.51.1665
Kobayashi-Saegusa, Haruhisa ; Iwamori, Satoru ; Sakaguchi, Reiko ; Sumita, Yoshihiro ; Shishido, Kazuo ; Noguchi, Norihisa. / Four Plasmids Simultaneously Maintained in Bacillus cereus, a Tetracycline-resistant Isolate. In: Agricultural and Biological Chemistry. 1987 ; Vol. 51, No. 6. pp. 1665-1670.
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