Fouling and cleaning of stainless steel surface: Adsorption and desorption behavior of bovine serum albumin and gelatin

Takaharu Sakiyama, Toshihisa Toyomasu, Asuka Nagata, Koreyoshi Imamura, Kazuhiro Nakanishi, Tokio Takahashi, Tadashi Nagai

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Adsorption and desorption behavior of bovine serum albumin (BSA) and gelatin on the surface of stainless steel particles are studied. The amount of BSA adsorbed increases significantly with temperature above 60°C whereas that of gelatin decreases slightly with increasing temperature. Results of adsorption experiments with S-carboxymethylated BSA show that the thermal aggregation of BSA molecules at the surface through intermolecular thiol-disulfide interchange reactions plays an important role in the adsorption of BSA at elevated temperatures. Furthermore, the initial desorption rate constants and residual amounts of the two proteins during caustic and enzymatic cleanings of the fouled particles are compared under various conditions. As a result, a large difference is found in the temperature dependence of the initial desorption rate constant in caustic cleaning, suggesting different modes of adsorption of the proteins.

Original languageEnglish
Pages (from-to)208-213
Number of pages6
JournalJournal of Chemical Engineering of Japan
Volume31
Issue number2
Publication statusPublished - 1998

Fingerprint

Stainless Steel
Gelatin
Bovine Serum Albumin
Fouling
Cleaning
Desorption
Stainless steel
Adsorption
Caustics
Rate constants
Proteins
Temperature
Interchanges
Agglomeration
Sulfhydryl Compounds
Disulfides
Molecules
Experiments

Keywords

  • Bovine Serum Albumin
  • Food Chemical Engineering
  • Fouling and Cleaning
  • Gelatin
  • Stainless Steel Surface

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)

Cite this

Fouling and cleaning of stainless steel surface : Adsorption and desorption behavior of bovine serum albumin and gelatin. / Sakiyama, Takaharu; Toyomasu, Toshihisa; Nagata, Asuka; Imamura, Koreyoshi; Nakanishi, Kazuhiro; Takahashi, Tokio; Nagai, Tadashi.

In: Journal of Chemical Engineering of Japan, Vol. 31, No. 2, 1998, p. 208-213.

Research output: Contribution to journalArticle

Sakiyama, T, Toyomasu, T, Nagata, A, Imamura, K, Nakanishi, K, Takahashi, T & Nagai, T 1998, 'Fouling and cleaning of stainless steel surface: Adsorption and desorption behavior of bovine serum albumin and gelatin', Journal of Chemical Engineering of Japan, vol. 31, no. 2, pp. 208-213.
Sakiyama, Takaharu ; Toyomasu, Toshihisa ; Nagata, Asuka ; Imamura, Koreyoshi ; Nakanishi, Kazuhiro ; Takahashi, Tokio ; Nagai, Tadashi. / Fouling and cleaning of stainless steel surface : Adsorption and desorption behavior of bovine serum albumin and gelatin. In: Journal of Chemical Engineering of Japan. 1998 ; Vol. 31, No. 2. pp. 208-213.
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