TY - JOUR
T1 - Formation of lens-like vesicles induced via microphase separations on a sorbitan monoester membrane with different headgroups
AU - Hayashi, Keita
AU - Iwai, Hideka
AU - Shimanouchi, Toshinori
AU - Umakoshi, Hiroshi
AU - Iwasaki, Tomoyuki
AU - Kato, Ayako
AU - Nakamura, Hidemi
N1 - Funding Information:
The authors thank Hiroki Kurata, Tetsuya Ogawa (Division of Electron Microscopy and Crystal Chemistry Institute for Chemical Research, Kyoto University), and Sakiko Fujita (Graduate School of Material Science, Nara Institute of Science and Technology) for their advice regarding cryo-TEM images. This research was supported by a Grant-in-Aid for Research Activity Start-up (No. 25889065), a Grant-in-Aid for Young Research (B) (No. 15K18279), a Grant-in-Aid for Scientific Research (A) (No. 26249116), and a Grant-in-Aid for Young Research (A) (No. 24686086) from the Japan Society for the Promotion of Science (JSPS) . This research was also supported by Kyoto University and the Nara Institute of Science and Technology Nano Technology Hub for a “Nanotechnology Platform Project,” sponsored by the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan .
Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2015/11/1
Y1 - 2015/11/1
N2 - The microphase separation of lipid molecules on a vesicle membrane can be induced, depending on the difference in the geometric structures of their headgroups. Through cryo-transmission-electron-microscopy analysis, a lens-like vesicle was prepared by mixing 50 wt% Span 40 (sorbitan monopalmitate) and 50 wt% Tween 40 [polyoxyethylene (20) sorbitan monopalmitate]. Considering the molecular structures of Span 40 and Tween 40, the high-curvature region was mainly formed by Tween 40. As determined by Fourier-transform infrared spectroscopy, dielectric-dispersion analysis, and differential scanning calorimetry, a hydration layer was likely formed because polyoxyethylene conjugates with the headgroups of Tween 40. These investigations of the obtained self-assembled aggregates of nonionic surfactants with heterogeneous surfaces could contribute to the development of new types of biomaterials.
AB - The microphase separation of lipid molecules on a vesicle membrane can be induced, depending on the difference in the geometric structures of their headgroups. Through cryo-transmission-electron-microscopy analysis, a lens-like vesicle was prepared by mixing 50 wt% Span 40 (sorbitan monopalmitate) and 50 wt% Tween 40 [polyoxyethylene (20) sorbitan monopalmitate]. Considering the molecular structures of Span 40 and Tween 40, the high-curvature region was mainly formed by Tween 40. As determined by Fourier-transform infrared spectroscopy, dielectric-dispersion analysis, and differential scanning calorimetry, a hydration layer was likely formed because polyoxyethylene conjugates with the headgroups of Tween 40. These investigations of the obtained self-assembled aggregates of nonionic surfactants with heterogeneous surfaces could contribute to the development of new types of biomaterials.
KW - Nonionic-surfactant vesicle
KW - Phase separation
KW - Self-assembled aggregate
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U2 - 10.1016/j.colsurfb.2015.07.071
DO - 10.1016/j.colsurfb.2015.07.071
M3 - Article
C2 - 26255167
AN - SCOPUS:84938767645
VL - 135
SP - 235
EP - 242
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
ER -