Formation of Filamentous Mn Oxide Particles by the Alphaproteobacterium Bosea sp. Strain BIWAKO-01

Seiko Furuta; Hisato Ikegaya; Hideki Hashimoto; Satoshi Ichise; Tetsuro Kohno; Naoyuki Miyata; Jun Takada

doi:10.1080/01490451.2014.982837

Formation of Filamentous Mn Oxide Particles by the Alphaproteobacterium Bosea sp. Strain BIWAKO-01

Seiko Furuta, Hisato Ikegaya, Hideki Hashimoto, Satoshi Ichise, Tetsuro Kohno, Naoyuki Miyata, Jun Takada

Faculty of Engineering

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Mn-rich filamentous particles present in stratified water environments are considered bacteriogenic; however, little is known about their causative agents. This study investigated the production of these particles by an alphaproteobacterium, Bosea sp. strain BIWAKO-01. Particle formation was promoted in static cultures with slightly viscous medium at pH 6.0−6.3 under low-O₂ conditions. The Mn(II) oxidation in cultures was slower in higher O₂ concentration. These results suggested that pH and O₂ concentration are important factors affecting filamentous Mn particle formation in the Mn(II) oxidizer. Lectin staining followed by fluorescence microscopy revealed the presence of specific carbohydrates in the filamentous structures. In addition, transmission electron microscopy, high angle annular dark field scanning transmission electron microscopy, and electron energy loss spectroscopy revealed the structural and spatial associations of Mn with O and C on a nanometer scale in filaments. The results suggested the occurrence of sheet-type Mn oxide likely due to the catalytic activity in exopolymeric substances including acidic polysaccharides.

Original language	English
Pages (from-to)	666-676
Number of pages	11
Journal	Geomicrobiology Journal
Volume	32
Issue number	8
DOIs	https://doi.org/10.1080/01490451.2014.982837
Publication status	Published - Sept 14 2015

Keywords

HAADF-STEM imaging and EELS mapping
Lake Biwa
Metallogenium
Mn(II)-oxidizing Bosea
biogenic Mn oxides

ASJC Scopus subject areas

Microbiology
Environmental Chemistry
Environmental Science(all)
Earth and Planetary Sciences (miscellaneous)

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10.1080/01490451.2014.982837

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title = "Formation of Filamentous Mn Oxide Particles by the Alphaproteobacterium Bosea sp. Strain BIWAKO-01",

abstract = "Mn-rich filamentous particles present in stratified water environments are considered bacteriogenic; however, little is known about their causative agents. This study investigated the production of these particles by an alphaproteobacterium, Bosea sp. strain BIWAKO-01. Particle formation was promoted in static cultures with slightly viscous medium at pH 6.0−6.3 under low-O2 conditions. The Mn(II) oxidation in cultures was slower in higher O2 concentration. These results suggested that pH and O2 concentration are important factors affecting filamentous Mn particle formation in the Mn(II) oxidizer. Lectin staining followed by fluorescence microscopy revealed the presence of specific carbohydrates in the filamentous structures. In addition, transmission electron microscopy, high angle annular dark field scanning transmission electron microscopy, and electron energy loss spectroscopy revealed the structural and spatial associations of Mn with O and C on a nanometer scale in filaments. The results suggested the occurrence of sheet-type Mn oxide likely due to the catalytic activity in exopolymeric substances including acidic polysaccharides.",

keywords = "HAADF-STEM imaging and EELS mapping, Lake Biwa, Metallogenium, Mn(II)-oxidizing Bosea, biogenic Mn oxides",

author = "Seiko Furuta and Hisato Ikegaya and Hideki Hashimoto and Satoshi Ichise and Tetsuro Kohno and Naoyuki Miyata and Jun Takada",

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AU - Furuta, Seiko

AU - Ikegaya, Hisato

AU - Hashimoto, Hideki

AU - Ichise, Satoshi

AU - Kohno, Tetsuro

AU - Miyata, Naoyuki

AU - Takada, Jun

PY - 2015/9/14

Y1 - 2015/9/14

N2 - Mn-rich filamentous particles present in stratified water environments are considered bacteriogenic; however, little is known about their causative agents. This study investigated the production of these particles by an alphaproteobacterium, Bosea sp. strain BIWAKO-01. Particle formation was promoted in static cultures with slightly viscous medium at pH 6.0−6.3 under low-O2 conditions. The Mn(II) oxidation in cultures was slower in higher O2 concentration. These results suggested that pH and O2 concentration are important factors affecting filamentous Mn particle formation in the Mn(II) oxidizer. Lectin staining followed by fluorescence microscopy revealed the presence of specific carbohydrates in the filamentous structures. In addition, transmission electron microscopy, high angle annular dark field scanning transmission electron microscopy, and electron energy loss spectroscopy revealed the structural and spatial associations of Mn with O and C on a nanometer scale in filaments. The results suggested the occurrence of sheet-type Mn oxide likely due to the catalytic activity in exopolymeric substances including acidic polysaccharides.

AB - Mn-rich filamentous particles present in stratified water environments are considered bacteriogenic; however, little is known about their causative agents. This study investigated the production of these particles by an alphaproteobacterium, Bosea sp. strain BIWAKO-01. Particle formation was promoted in static cultures with slightly viscous medium at pH 6.0−6.3 under low-O2 conditions. The Mn(II) oxidation in cultures was slower in higher O2 concentration. These results suggested that pH and O2 concentration are important factors affecting filamentous Mn particle formation in the Mn(II) oxidizer. Lectin staining followed by fluorescence microscopy revealed the presence of specific carbohydrates in the filamentous structures. In addition, transmission electron microscopy, high angle annular dark field scanning transmission electron microscopy, and electron energy loss spectroscopy revealed the structural and spatial associations of Mn with O and C on a nanometer scale in filaments. The results suggested the occurrence of sheet-type Mn oxide likely due to the catalytic activity in exopolymeric substances including acidic polysaccharides.

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KW - Metallogenium

KW - Mn(II)-oxidizing Bosea

KW - biogenic Mn oxides

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Formation of Filamentous Mn Oxide Particles by the Alphaproteobacterium Bosea sp. Strain BIWAKO-01

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