Formation of Filamentous Mn Oxide Particles by the Alphaproteobacterium Bosea sp. Strain BIWAKO-01

Seiko Furuta, Hisato Ikegaya, Hideki Hashimoto, Satoshi Ichise, Tetsuro Kohno, Naoyuki Miyata, Jun Takada

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Mn-rich filamentous particles present in stratified water environments are considered bacteriogenic; however, little is known about their causative agents. This study investigated the production of these particles by an alphaproteobacterium, Bosea sp. strain BIWAKO-01. Particle formation was promoted in static cultures with slightly viscous medium at pH 6.0−6.3 under low-O2 conditions. The Mn(II) oxidation in cultures was slower in higher O2 concentration. These results suggested that pH and O2 concentration are important factors affecting filamentous Mn particle formation in the Mn(II) oxidizer. Lectin staining followed by fluorescence microscopy revealed the presence of specific carbohydrates in the filamentous structures. In addition, transmission electron microscopy, high angle annular dark field scanning transmission electron microscopy, and electron energy loss spectroscopy revealed the structural and spatial associations of Mn with O and C on a nanometer scale in filaments. The results suggested the occurrence of sheet-type Mn oxide likely due to the catalytic activity in exopolymeric substances including acidic polysaccharides.

Original languageEnglish
Pages (from-to)666-676
Number of pages11
JournalGeomicrobiology Journal
Volume32
Issue number8
DOIs
Publication statusPublished - Sep 14 2015

Fingerprint

Alphaproteobacteria
Oxides
Electron Energy-Loss Spectroscopy
oxide
Transmission electron microscopy
Scanning Transmission Electron Microscopy
Electron energy loss spectroscopy
Fluorescence microscopy
Transmission Electron Microscopy
Fluorescence Microscopy
Lectins
Polysaccharides
transmission electron microscopy
Catalyst activity
Carbohydrates
Staining and Labeling
Oxidation
Scanning electron microscopy
Water
polysaccharide

Keywords

  • biogenic Mn oxides
  • HAADF-STEM imaging and EELS mapping
  • Lake Biwa
  • Metallogenium
  • Mn(II)-oxidizing Bosea

ASJC Scopus subject areas

  • Earth and Planetary Sciences (miscellaneous)
  • Microbiology
  • Environmental Science(all)
  • Environmental Chemistry

Cite this

Furuta, S., Ikegaya, H., Hashimoto, H., Ichise, S., Kohno, T., Miyata, N., & Takada, J. (2015). Formation of Filamentous Mn Oxide Particles by the Alphaproteobacterium Bosea sp. Strain BIWAKO-01. Geomicrobiology Journal, 32(8), 666-676. https://doi.org/10.1080/01490451.2014.982837

Formation of Filamentous Mn Oxide Particles by the Alphaproteobacterium Bosea sp. Strain BIWAKO-01. / Furuta, Seiko; Ikegaya, Hisato; Hashimoto, Hideki; Ichise, Satoshi; Kohno, Tetsuro; Miyata, Naoyuki; Takada, Jun.

In: Geomicrobiology Journal, Vol. 32, No. 8, 14.09.2015, p. 666-676.

Research output: Contribution to journalArticle

Furuta, S, Ikegaya, H, Hashimoto, H, Ichise, S, Kohno, T, Miyata, N & Takada, J 2015, 'Formation of Filamentous Mn Oxide Particles by the Alphaproteobacterium Bosea sp. Strain BIWAKO-01', Geomicrobiology Journal, vol. 32, no. 8, pp. 666-676. https://doi.org/10.1080/01490451.2014.982837
Furuta, Seiko ; Ikegaya, Hisato ; Hashimoto, Hideki ; Ichise, Satoshi ; Kohno, Tetsuro ; Miyata, Naoyuki ; Takada, Jun. / Formation of Filamentous Mn Oxide Particles by the Alphaproteobacterium Bosea sp. Strain BIWAKO-01. In: Geomicrobiology Journal. 2015 ; Vol. 32, No. 8. pp. 666-676.
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