Formation of advanced glycosylation end products and oxidative stress in young patients with type 1 diabetes

Hirokazu Tsukahara, Kyouichi Sekine, Mayumi Uchiyama, Hisako Kawakami, Ikue Hata, Yukiko Todoroki, Masahiro Hiraoka, Masayuki Kaji, Tohru Yorifuji, Toru Momoi, Kazuhiro Yoshihara, Masatoshi Beppu, Mitsufumi Mayumi

Research output: Contribution to journalArticle

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Abstract

Increased production of advanced glycosylation end products (AGEs) and augmented oxidative stress may contribute to vascular complications in diabetes. Little is known about the formation and accumulation of AGEs in young patients with type 1 diabetes. The aim of the present study was to investigate whether AGE production and oxidative stress are augmented in young patients with type 1 diabetes at early clinical stages of the disease. Urine samples of 38 patients with type 1 diabetes [mean age (±SD), 12.8 ± 4.5 y; diabetes duration, 5.7 ± 4.3 y; HbA1c, 8.0 ± 1.6%; urinary albumin excretion, 12.6 ± 14.4 mg/g creatinine (Cr)] and those of 60 age-matched healthy control subjects were assayed for AGEs, pentosidine and pyrraline, and markers of oxidative stress, 8-hydroxy-2′-deoxyguanosine (8-OHdG) and acrolein-lysine. Of these four markers, urinary concentrations of pentosidine, 8-OHdG, and acrolein-lysine were significantly higher in the patients with diabetes than in the healthy control subjects. For the patient group, pentosidine correlated significantly with 8-OHdG and acrolein-lysine, and pyrraline correlated significantly with acrolein-lysine. Urinary pentosidine, 8-OHdG, and acrolein-lysine but not pyrraline correlated significantly with urinary albumin excretion. Patients with microalbuminuria (≥15 mg/g Cr) showed significantly higher levels of all four markers than did normoalbuminuric patients and control subjects. The present study indicates that accumulation of AGEs, whose formation is closely linked to oxidative stress, and resultant endothelial dysfunction may start early in the course of type 1 diabetes. This means that the risk of vascular complications may be present at an early age and that the best possible glycemic control should be emphasized from the diagnosis of diabetes.

Original languageEnglish
Pages (from-to)419-424
Number of pages6
JournalPediatric Research
Volume54
Issue number3
DOIs
Publication statusPublished - Sep 1 2003
Externally publishedYes

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Advanced Glycosylation End Products
Type 1 Diabetes Mellitus
Acrolein
Oxidative Stress
Lysine
Blood Vessels
Albumins
Creatinine
Healthy Volunteers
Diabetes Complications
Urine
pentosidine
2-formyl-5-(hydroxymethyl)pyrrole-1-norleucine

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Formation of advanced glycosylation end products and oxidative stress in young patients with type 1 diabetes. / Tsukahara, Hirokazu; Sekine, Kyouichi; Uchiyama, Mayumi; Kawakami, Hisako; Hata, Ikue; Todoroki, Yukiko; Hiraoka, Masahiro; Kaji, Masayuki; Yorifuji, Tohru; Momoi, Toru; Yoshihara, Kazuhiro; Beppu, Masatoshi; Mayumi, Mitsufumi.

In: Pediatric Research, Vol. 54, No. 3, 01.09.2003, p. 419-424.

Research output: Contribution to journalArticle

Tsukahara, H, Sekine, K, Uchiyama, M, Kawakami, H, Hata, I, Todoroki, Y, Hiraoka, M, Kaji, M, Yorifuji, T, Momoi, T, Yoshihara, K, Beppu, M & Mayumi, M 2003, 'Formation of advanced glycosylation end products and oxidative stress in young patients with type 1 diabetes', Pediatric Research, vol. 54, no. 3, pp. 419-424. https://doi.org/10.1203/01.PDR.0000076662.72100.74
Tsukahara, Hirokazu ; Sekine, Kyouichi ; Uchiyama, Mayumi ; Kawakami, Hisako ; Hata, Ikue ; Todoroki, Yukiko ; Hiraoka, Masahiro ; Kaji, Masayuki ; Yorifuji, Tohru ; Momoi, Toru ; Yoshihara, Kazuhiro ; Beppu, Masatoshi ; Mayumi, Mitsufumi. / Formation of advanced glycosylation end products and oxidative stress in young patients with type 1 diabetes. In: Pediatric Research. 2003 ; Vol. 54, No. 3. pp. 419-424.
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