Formation and resolution of ankylosis under application of recombinant human bone morphogenetic protein-2 (rhBMP-2) to class III furcation defects in cats

Dairo Takahashi, T. Odajima, M. Morita, M. Kawanami, H. Kato

Research output: Contribution to journalReview article

25 Citations (Scopus)


Objectives: Periodontal regeneration under application of bone morphogenetic protein (BMP) is compromised by ankylosis. Ankylosis disappearance following application of BMP has been observed in the case of a small defect, which might be beneficial change for periodontal regeneration. However, the histological observation of ankylosis disappearance has not been demonstrated in a large defect. The purpose of this present study was to confirm resolution of ankylosis during periodontal regeneration by recombinant human BMP-2 (rhBMP-2) applied to class III furcation defects. Material and methods: Class III furcation defects were created in the premolars of six adult cats. The rhBMP-2 material, prepared by applying rhBMP-2 to a combination of polylactic acid-polygricolic copolymer and gelatin sponge (PGS; 0.33 μg rhBMP-2/mm 3 PGS) or control material containing only PGS, was implanted into each defect. The cats were killed at 3, 6 or 12 weeks after surgery and serial sections were prepared for histological and histometrical observation. Results: Ankylosis was observed in some of the rhBMP-2/PGS group at 3 and 6 weeks, but not at 12 weeks. At 6 weeks, osteoclast-like cells were visible in the rhBMP-2/PGS group with ankylosis. Residual PGS was evident between the bone and root surface in the rhBMP-2/PGS group without ankylosis at 3 weeks. Conclusions: Resolution of ankylosis by osteoclast-like cells possibly occurred under application of rhBMP-2. Residual PGS might play an important role in preventing ankylosis formation.

Original languageEnglish
Pages (from-to)299-305
Number of pages7
JournalJournal of Periodontal Research
Issue number4
Publication statusPublished - Aug 1 2005
Externally publishedYes



  • Osteoclast-like cell
  • Recombinant human bone morphogenetic protein-2
  • Residual polylactic acid-polygricolic copolymer and gelatin sponge
  • Resolution of ankylosis

ASJC Scopus subject areas

  • Periodontics

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