Formation and resolution of ankylosis under application of recombinant human bone morphogenetic protein-2 (rhBMP-2) to class III furcation defects in cats

Dairo Takahashi, T. Odajima, Manabu Morita, M. Kawanami, H. Kato

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Objectives: Periodontal regeneration under application of bone morphogenetic protein (BMP) is compromised by ankylosis. Ankylosis disappearance following application of BMP has been observed in the case of a small defect, which might be beneficial change for periodontal regeneration. However, the histological observation of ankylosis disappearance has not been demonstrated in a large defect. The purpose of this present study was to confirm resolution of ankylosis during periodontal regeneration by recombinant human BMP-2 (rhBMP-2) applied to class III furcation defects. Material and methods: Class III furcation defects were created in the premolars of six adult cats. The rhBMP-2 material, prepared by applying rhBMP-2 to a combination of polylactic acid-polygricolic copolymer and gelatin sponge (PGS; 0.33 μg rhBMP-2/mm 3 PGS) or control material containing only PGS, was implanted into each defect. The cats were killed at 3, 6 or 12 weeks after surgery and serial sections were prepared for histological and histometrical observation. Results: Ankylosis was observed in some of the rhBMP-2/PGS group at 3 and 6 weeks, but not at 12 weeks. At 6 weeks, osteoclast-like cells were visible in the rhBMP-2/PGS group with ankylosis. Residual PGS was evident between the bone and root surface in the rhBMP-2/PGS group without ankylosis at 3 weeks. Conclusions: Resolution of ankylosis by osteoclast-like cells possibly occurred under application of rhBMP-2. Residual PGS might play an important role in preventing ankylosis formation.

Original languageEnglish
Pages (from-to)299-305
Number of pages7
JournalJournal of Periodontal Research
Volume40
Issue number4
DOIs
Publication statusPublished - Aug 2005
Externally publishedYes

Fingerprint

Furcation Defects
Ankylosis
Cats
Regeneration
Bone Morphogenetic Proteins
Osteoclasts
Observation
recombinant human bone morphogenetic protein-2
Bicuspid
Porifera
Gelatin

Keywords

  • Osteoclast-like cell
  • Recombinant human bone morphogenetic protein-2
  • Residual polylactic acid-polygricolic copolymer and gelatin sponge
  • Resolution of ankylosis

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Formation and resolution of ankylosis under application of recombinant human bone morphogenetic protein-2 (rhBMP-2) to class III furcation defects in cats. / Takahashi, Dairo; Odajima, T.; Morita, Manabu; Kawanami, M.; Kato, H.

In: Journal of Periodontal Research, Vol. 40, No. 4, 08.2005, p. 299-305.

Research output: Contribution to journalArticle

@article{5413301069074d2fa32c255cf646da77,
title = "Formation and resolution of ankylosis under application of recombinant human bone morphogenetic protein-2 (rhBMP-2) to class III furcation defects in cats",
abstract = "Objectives: Periodontal regeneration under application of bone morphogenetic protein (BMP) is compromised by ankylosis. Ankylosis disappearance following application of BMP has been observed in the case of a small defect, which might be beneficial change for periodontal regeneration. However, the histological observation of ankylosis disappearance has not been demonstrated in a large defect. The purpose of this present study was to confirm resolution of ankylosis during periodontal regeneration by recombinant human BMP-2 (rhBMP-2) applied to class III furcation defects. Material and methods: Class III furcation defects were created in the premolars of six adult cats. The rhBMP-2 material, prepared by applying rhBMP-2 to a combination of polylactic acid-polygricolic copolymer and gelatin sponge (PGS; 0.33 μg rhBMP-2/mm 3 PGS) or control material containing only PGS, was implanted into each defect. The cats were killed at 3, 6 or 12 weeks after surgery and serial sections were prepared for histological and histometrical observation. Results: Ankylosis was observed in some of the rhBMP-2/PGS group at 3 and 6 weeks, but not at 12 weeks. At 6 weeks, osteoclast-like cells were visible in the rhBMP-2/PGS group with ankylosis. Residual PGS was evident between the bone and root surface in the rhBMP-2/PGS group without ankylosis at 3 weeks. Conclusions: Resolution of ankylosis by osteoclast-like cells possibly occurred under application of rhBMP-2. Residual PGS might play an important role in preventing ankylosis formation.",
keywords = "Osteoclast-like cell, Recombinant human bone morphogenetic protein-2, Residual polylactic acid-polygricolic copolymer and gelatin sponge, Resolution of ankylosis",
author = "Dairo Takahashi and T. Odajima and Manabu Morita and M. Kawanami and H. Kato",
year = "2005",
month = "8",
doi = "10.1111/j.1600-0765.2005.00794.x",
language = "English",
volume = "40",
pages = "299--305",
journal = "Journal of Periodontal Research",
issn = "0022-3484",
publisher = "Blackwell Munksgaard",
number = "4",

}

TY - JOUR

T1 - Formation and resolution of ankylosis under application of recombinant human bone morphogenetic protein-2 (rhBMP-2) to class III furcation defects in cats

AU - Takahashi, Dairo

AU - Odajima, T.

AU - Morita, Manabu

AU - Kawanami, M.

AU - Kato, H.

PY - 2005/8

Y1 - 2005/8

N2 - Objectives: Periodontal regeneration under application of bone morphogenetic protein (BMP) is compromised by ankylosis. Ankylosis disappearance following application of BMP has been observed in the case of a small defect, which might be beneficial change for periodontal regeneration. However, the histological observation of ankylosis disappearance has not been demonstrated in a large defect. The purpose of this present study was to confirm resolution of ankylosis during periodontal regeneration by recombinant human BMP-2 (rhBMP-2) applied to class III furcation defects. Material and methods: Class III furcation defects were created in the premolars of six adult cats. The rhBMP-2 material, prepared by applying rhBMP-2 to a combination of polylactic acid-polygricolic copolymer and gelatin sponge (PGS; 0.33 μg rhBMP-2/mm 3 PGS) or control material containing only PGS, was implanted into each defect. The cats were killed at 3, 6 or 12 weeks after surgery and serial sections were prepared for histological and histometrical observation. Results: Ankylosis was observed in some of the rhBMP-2/PGS group at 3 and 6 weeks, but not at 12 weeks. At 6 weeks, osteoclast-like cells were visible in the rhBMP-2/PGS group with ankylosis. Residual PGS was evident between the bone and root surface in the rhBMP-2/PGS group without ankylosis at 3 weeks. Conclusions: Resolution of ankylosis by osteoclast-like cells possibly occurred under application of rhBMP-2. Residual PGS might play an important role in preventing ankylosis formation.

AB - Objectives: Periodontal regeneration under application of bone morphogenetic protein (BMP) is compromised by ankylosis. Ankylosis disappearance following application of BMP has been observed in the case of a small defect, which might be beneficial change for periodontal regeneration. However, the histological observation of ankylosis disappearance has not been demonstrated in a large defect. The purpose of this present study was to confirm resolution of ankylosis during periodontal regeneration by recombinant human BMP-2 (rhBMP-2) applied to class III furcation defects. Material and methods: Class III furcation defects were created in the premolars of six adult cats. The rhBMP-2 material, prepared by applying rhBMP-2 to a combination of polylactic acid-polygricolic copolymer and gelatin sponge (PGS; 0.33 μg rhBMP-2/mm 3 PGS) or control material containing only PGS, was implanted into each defect. The cats were killed at 3, 6 or 12 weeks after surgery and serial sections were prepared for histological and histometrical observation. Results: Ankylosis was observed in some of the rhBMP-2/PGS group at 3 and 6 weeks, but not at 12 weeks. At 6 weeks, osteoclast-like cells were visible in the rhBMP-2/PGS group with ankylosis. Residual PGS was evident between the bone and root surface in the rhBMP-2/PGS group without ankylosis at 3 weeks. Conclusions: Resolution of ankylosis by osteoclast-like cells possibly occurred under application of rhBMP-2. Residual PGS might play an important role in preventing ankylosis formation.

KW - Osteoclast-like cell

KW - Recombinant human bone morphogenetic protein-2

KW - Residual polylactic acid-polygricolic copolymer and gelatin sponge

KW - Resolution of ankylosis

UR - http://www.scopus.com/inward/record.url?scp=22144489185&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=22144489185&partnerID=8YFLogxK

U2 - 10.1111/j.1600-0765.2005.00794.x

DO - 10.1111/j.1600-0765.2005.00794.x

M3 - Article

VL - 40

SP - 299

EP - 305

JO - Journal of Periodontal Research

JF - Journal of Periodontal Research

SN - 0022-3484

IS - 4

ER -