Abstract
Formation of the lateral flagella (L flagella) of V. parahaemolyticus was studied immunologically, using specific antiserum against L flagella. On solid medium, L flagella were formed at both high (37°C) and low (25° temperatures, although at high temperatures they became dissociated from the cells and decomposed in the medium. L. flagella were not formed in liquid or soft agar medium. Formation of L flagella was decreased by lowering the pH of the medium and repressed by transferring the cells from solid medium to liquid medium. Mutants possessing L flagella but not a polar monotrichous flagellum (M flagellum) swarmed on solid medium, whereas mutants possessing an M flagellum but not L flagella did not. When the former mutants were grown on solid medium and then transferred to liquid medium, the cells oscillated until they lost L flagella. It is postulated that L flagella are locomotive organelles on solid medium and in some cases also in liquid medium, whereas M flagella are locomotive organelles only in liquid medium.
| Original language | English |
|---|---|
| Pages (from-to) | 1266-1271 |
| Number of pages | 6 |
| Journal | Journal of Bacteriology |
| Volume | 129 |
| Issue number | 3 |
| Publication status | Published - 1977 |
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ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Immunology
Cite this
Formation and function of Vibrio parahaemolyticus lateral flagella. / Shinoda, S.; Okamoto, Keinosuke.
In: Journal of Bacteriology, Vol. 129, No. 3, 1977, p. 1266-1271.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Formation and function of Vibrio parahaemolyticus lateral flagella
AU - Shinoda, S.
AU - Okamoto, Keinosuke
PY - 1977
Y1 - 1977
N2 - Formation of the lateral flagella (L flagella) of V. parahaemolyticus was studied immunologically, using specific antiserum against L flagella. On solid medium, L flagella were formed at both high (37°C) and low (25° temperatures, although at high temperatures they became dissociated from the cells and decomposed in the medium. L. flagella were not formed in liquid or soft agar medium. Formation of L flagella was decreased by lowering the pH of the medium and repressed by transferring the cells from solid medium to liquid medium. Mutants possessing L flagella but not a polar monotrichous flagellum (M flagellum) swarmed on solid medium, whereas mutants possessing an M flagellum but not L flagella did not. When the former mutants were grown on solid medium and then transferred to liquid medium, the cells oscillated until they lost L flagella. It is postulated that L flagella are locomotive organelles on solid medium and in some cases also in liquid medium, whereas M flagella are locomotive organelles only in liquid medium.
AB - Formation of the lateral flagella (L flagella) of V. parahaemolyticus was studied immunologically, using specific antiserum against L flagella. On solid medium, L flagella were formed at both high (37°C) and low (25° temperatures, although at high temperatures they became dissociated from the cells and decomposed in the medium. L. flagella were not formed in liquid or soft agar medium. Formation of L flagella was decreased by lowering the pH of the medium and repressed by transferring the cells from solid medium to liquid medium. Mutants possessing L flagella but not a polar monotrichous flagellum (M flagellum) swarmed on solid medium, whereas mutants possessing an M flagellum but not L flagella did not. When the former mutants were grown on solid medium and then transferred to liquid medium, the cells oscillated until they lost L flagella. It is postulated that L flagella are locomotive organelles on solid medium and in some cases also in liquid medium, whereas M flagella are locomotive organelles only in liquid medium.
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M3 - Article
VL - 129
SP - 1266
EP - 1271
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 3
ER -