Form of aluminium for uptake and translocation in buckwheat (Fagopyrum esculentum Moench)

Jian Feng Ma, Syuntaro Hiradate

Research output: Contribution to journalArticle

144 Citations (Scopus)

Abstract

The forms of Al for uptake by the roots and translocation from the root to the shoot were investigated in a buckwheat (Fagopyrum esculentum Moench, cv. Jianxi) that accumulates Al in its leaves. The Al concentration in the xylem sap was 15-fold higher in the plants exposed to AlCl3 than in those exposed to an Al-oxalate (1:3) complex, suggesting that the roots take up Al in the ionic form. The Al concentration in the xylem sap was 4-fold higher than that in the external solution after a 1-h exposure to AlCl3 solution and 10-fold higher after a 2-h exposure. The Al concentration in the xylem sap increased with increasing Al concentration in the external solution. The Al uptake was not affected by a respiratory inhibitor, hydroxylamine, but significantly inhibited by the addition of La. These results suggest that Al uptake by the root is a passive process, and La3+ competes for the binding sites for Al3+ on the plasma membrane. The form of Al in the xylem sap was identified by 27Al-nuclear magnetic resonance analysis. The chemical shift of 27Al in the xylem sap was around 10.9 ppm, which is consistent with that of the Al-citrate complex. Furthermore, the dominant organic acid in the xylem sap was citric acid, indicating that Al was translocated in the form of Al-citrate complex. Because Al is present as Al-oxalate (1:3) in the root, the present data show that ligand exchange from oxalate to citrate occurs before Al is released to xylem.

Original languageEnglish
Pages (from-to)355-360
Number of pages6
JournalPlanta
Volume211
Issue number3
DOIs
Publication statusPublished - Jan 1 2000
Externally publishedYes

Keywords

  • Aluminium form
  • Fagopyrum (Al uptake)
  • Root (Al uptake)
  • Translocation (Al)
  • Uptake (Al)
  • Xylem sap

ASJC Scopus subject areas

  • Genetics
  • Plant Science

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