Form of aluminium for uptake and translocation in buckwheat (Fagopyrum esculentum Moench)

Jian Feng Ma, Syuntaro Hiradate

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

The forms of Al for uptake by the roots and translocation from the root to the shoot were investigated in a buckwheat (Fagopyrum esculentum Moench, cv. Jianxi) that accumulates Al in its leaves. The Al concentration in the xylem sap was 15-fold higher in the plants exposed to AlCl3 than in those exposed to an Al-oxalate (1:3) complex, suggesting that the roots take up Al in the ionic form. The Al concentration in the xylem sap was 4-fold higher than that in the external solution after a 1-h exposure to AlCl3 solution and 10-fold higher after a 2-h exposure. The Al concentration in the xylem sap increased with increasing Al concentration in the external solution. The Al uptake was not affected by a respiratory inhibitor, hydroxylamine, but significantly inhibited by the addition of La. These results suggest that Al uptake by the root is a passive process, and La3+ competes for the binding sites for Al3+ on the plasma membrane. The form of Al in the xylem sap was identified by 27Al-nuclear magnetic resonance analysis. The chemical shift of 27Al in the xylem sap was around 10.9 ppm, which is consistent with that of the Al-citrate complex. Furthermore, the dominant organic acid in the xylem sap was citric acid, indicating that Al was translocated in the form of Al-citrate complex. Because Al is present as Al-oxalate (1:3) in the root, the present data show that ligand exchange from oxalate to citrate occurs before Al is released to xylem.

Original languageEnglish
Pages (from-to)355-360
Number of pages6
JournalPlanta
Volume211
Issue number3
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Fagopyrum
Xylem
Fagopyrum esculentum
buckwheat
Aluminum
xylem
aluminum
sap
Citric Acid
Oxalates
oxalates
citrates
Hydroxylamine
citric acid
organic acids and salts
nuclear magnetic resonance spectroscopy
binding sites
Magnetic Resonance Spectroscopy
plasma membrane
Binding Sites

Keywords

  • Aluminium form
  • Fagopyrum (Al uptake)
  • Root (Al uptake)
  • Translocation (Al)
  • Uptake (Al)
  • Xylem sap

ASJC Scopus subject areas

  • Plant Science

Cite this

Form of aluminium for uptake and translocation in buckwheat (Fagopyrum esculentum Moench). / Ma, Jian Feng; Hiradate, Syuntaro.

In: Planta, Vol. 211, No. 3, 2000, p. 355-360.

Research output: Contribution to journalArticle

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