Forebrain ischemia-induced nitric oxide synthase (iNOS) induction, neuronal apoptosis and drug action in rats

Yasuyuki Nomura, Tomohiro Miyasaka, Itsuko Baba, Takashi Uehara

Research output: Contribution to journalArticle

Abstract

We examined the induction of nitric oxide synthase (iNOS) mRNA and neuronal death after forebrain ischemia by 4 vessel occlusion in rat hippocampus. In addition, we tried to characterize whether or not the delayed neuronal cell death is apoptosis or necrosis by means of TUNEL method and staining of chromatin dye, Hoechst 33258. Nuclei of the CA 1 pyramidal neurons were positive to TUNEL after ischemic insult, but not at the preceding stage. These results suggest that the delayed neuronal death following forebrain ischemia is apoptosis. Moreover, induction of iNOS mRNA was detected 3 days after ischemic insult in hippocampus. It seems to be a close causality between he neuronal death following transient ischemia and iNOS/NO in the hippocampus. We then found that herbimycin A, a potent tyrosine kinase inhibitor, and aspirin inhibited LPS/IFN-γ-induced iNOS induction in C6 glioma cells. It is interesting to examine whether or not both drugs inhibit brain ischemia-induced neuronal apoptosis. We presented and discussed the mechanism of NO-induced apoptosis using NG 10815 cells too.

Original languageEnglish
JournalFolia Pharmacologica Japonica
Volume108
Issue numberSUPPL. 1
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Nitric Oxide Synthase Type I
Prosencephalon
Ischemia
Nitric Oxide Synthase
Apoptosis
Hippocampus
In Situ Nick-End Labeling
Pharmaceutical Preparations
Bisbenzimidazole
Messenger RNA
Pyramidal Cells
Brain Ischemia
Glioma
Causality
Protein-Tyrosine Kinases
Aspirin
Chromatin
Cell Death
Necrosis
Coloring Agents

ASJC Scopus subject areas

  • Pharmacology

Cite this

Forebrain ischemia-induced nitric oxide synthase (iNOS) induction, neuronal apoptosis and drug action in rats. / Nomura, Yasuyuki; Miyasaka, Tomohiro; Baba, Itsuko; Uehara, Takashi.

In: Folia Pharmacologica Japonica, Vol. 108, No. SUPPL. 1, 1996.

Research output: Contribution to journalArticle

@article{b4db58a972144d89b0691eb8b60893f9,
title = "Forebrain ischemia-induced nitric oxide synthase (iNOS) induction, neuronal apoptosis and drug action in rats",
abstract = "We examined the induction of nitric oxide synthase (iNOS) mRNA and neuronal death after forebrain ischemia by 4 vessel occlusion in rat hippocampus. In addition, we tried to characterize whether or not the delayed neuronal cell death is apoptosis or necrosis by means of TUNEL method and staining of chromatin dye, Hoechst 33258. Nuclei of the CA 1 pyramidal neurons were positive to TUNEL after ischemic insult, but not at the preceding stage. These results suggest that the delayed neuronal death following forebrain ischemia is apoptosis. Moreover, induction of iNOS mRNA was detected 3 days after ischemic insult in hippocampus. It seems to be a close causality between he neuronal death following transient ischemia and iNOS/NO in the hippocampus. We then found that herbimycin A, a potent tyrosine kinase inhibitor, and aspirin inhibited LPS/IFN-γ-induced iNOS induction in C6 glioma cells. It is interesting to examine whether or not both drugs inhibit brain ischemia-induced neuronal apoptosis. We presented and discussed the mechanism of NO-induced apoptosis using NG 10815 cells too.",
author = "Yasuyuki Nomura and Tomohiro Miyasaka and Itsuko Baba and Takashi Uehara",
year = "1996",
language = "English",
volume = "108",
journal = "Folia Pharmacologica Japonica",
issn = "0015-5691",
publisher = "Japanese Pharmacological Society",
number = "SUPPL. 1",

}

TY - JOUR

T1 - Forebrain ischemia-induced nitric oxide synthase (iNOS) induction, neuronal apoptosis and drug action in rats

AU - Nomura, Yasuyuki

AU - Miyasaka, Tomohiro

AU - Baba, Itsuko

AU - Uehara, Takashi

PY - 1996

Y1 - 1996

N2 - We examined the induction of nitric oxide synthase (iNOS) mRNA and neuronal death after forebrain ischemia by 4 vessel occlusion in rat hippocampus. In addition, we tried to characterize whether or not the delayed neuronal cell death is apoptosis or necrosis by means of TUNEL method and staining of chromatin dye, Hoechst 33258. Nuclei of the CA 1 pyramidal neurons were positive to TUNEL after ischemic insult, but not at the preceding stage. These results suggest that the delayed neuronal death following forebrain ischemia is apoptosis. Moreover, induction of iNOS mRNA was detected 3 days after ischemic insult in hippocampus. It seems to be a close causality between he neuronal death following transient ischemia and iNOS/NO in the hippocampus. We then found that herbimycin A, a potent tyrosine kinase inhibitor, and aspirin inhibited LPS/IFN-γ-induced iNOS induction in C6 glioma cells. It is interesting to examine whether or not both drugs inhibit brain ischemia-induced neuronal apoptosis. We presented and discussed the mechanism of NO-induced apoptosis using NG 10815 cells too.

AB - We examined the induction of nitric oxide synthase (iNOS) mRNA and neuronal death after forebrain ischemia by 4 vessel occlusion in rat hippocampus. In addition, we tried to characterize whether or not the delayed neuronal cell death is apoptosis or necrosis by means of TUNEL method and staining of chromatin dye, Hoechst 33258. Nuclei of the CA 1 pyramidal neurons were positive to TUNEL after ischemic insult, but not at the preceding stage. These results suggest that the delayed neuronal death following forebrain ischemia is apoptosis. Moreover, induction of iNOS mRNA was detected 3 days after ischemic insult in hippocampus. It seems to be a close causality between he neuronal death following transient ischemia and iNOS/NO in the hippocampus. We then found that herbimycin A, a potent tyrosine kinase inhibitor, and aspirin inhibited LPS/IFN-γ-induced iNOS induction in C6 glioma cells. It is interesting to examine whether or not both drugs inhibit brain ischemia-induced neuronal apoptosis. We presented and discussed the mechanism of NO-induced apoptosis using NG 10815 cells too.

UR - http://www.scopus.com/inward/record.url?scp=0029831815&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029831815&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0029831815

VL - 108

JO - Folia Pharmacologica Japonica

JF - Folia Pharmacologica Japonica

SN - 0015-5691

IS - SUPPL. 1

ER -