Food preservative potential of gassericin A-containing concentrate prepared from cheese whey culture supernatant of Lactobacillus gasseri LA39

Kiyoshi Nakamura, Kensuke Arakawa, Yasushi Kawai, Narimi Yasuta, Takahiro Chujo, Masamichi Watanabe, Hiroyuki Iioka, Masashi Tanioka, Junko Nishimura, Haruki Kitazawa, Koichi Tsurumi, Tadao Saito

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Gassericin A (GA) is a circular bacteriocin produced by Lactobacillus gasseri LA39. In this study, GA-containing concentrate was prepared using a cross-flow membrane filtration device (30kDa cut-off) from the culture supernatant of Lb.gasseri LA39 cultivated in a cheese whey-based food-grade medium. The bacteriocin activity titer in the concentrate was 16 times as high as that of the culture supernatant and was completely maintained through each incubation at 4°C for 3months, 37°C for 2months, 60°C for 5 h, and 100°C for 30min. The GA-containing concentrate was used with glycine powder to make custard creams, and then four representative strains of custard cream spoilage bacteria (Bacillus cereus, Lactococcus lactis subsp. lactis, Achromobacter denitrificans and Pseudomonas fluorescens) were individually inoculated at c. 103 colony forming units/g in the custard creams. Throughout 30days of incubation at 30°C, all of the inoculated bacteria were completely inhibited by the combination of 5% (w/w) of the GA-containing concentrate and 0.5% (w/w) glycine. This is the first highly practical application of GA to foods as a biopreservative, and the concentration method and the bacteriocin concentrate would contribute to biopreservation of several foods.

Original languageEnglish
Pages (from-to)144-149
Number of pages6
JournalAnimal Science Journal
Volume84
Issue number2
DOIs
Publication statusPublished - Feb 2013

Fingerprint

Food Preservatives
Lactobacillus gasseri
cheese whey
food preservatives
Cheese
custards
concentrates
Bacteriocins
bacteriocins
cream
Food
Glycine
food biopreservation
Achromobacter denitrificans
Achromobacter
biopreservatives
Bacteria
Lactococcus lactis subsp. lactis
Pseudomonas fluorescens
Lactococcus lactis

Keywords

  • Bacteriocin
  • Biopreservation
  • Custard cream
  • Gassericin A
  • Whey

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Food preservative potential of gassericin A-containing concentrate prepared from cheese whey culture supernatant of Lactobacillus gasseri LA39. / Nakamura, Kiyoshi; Arakawa, Kensuke; Kawai, Yasushi; Yasuta, Narimi; Chujo, Takahiro; Watanabe, Masamichi; Iioka, Hiroyuki; Tanioka, Masashi; Nishimura, Junko; Kitazawa, Haruki; Tsurumi, Koichi; Saito, Tadao.

In: Animal Science Journal, Vol. 84, No. 2, 02.2013, p. 144-149.

Research output: Contribution to journalArticle

Nakamura, K, Arakawa, K, Kawai, Y, Yasuta, N, Chujo, T, Watanabe, M, Iioka, H, Tanioka, M, Nishimura, J, Kitazawa, H, Tsurumi, K & Saito, T 2013, 'Food preservative potential of gassericin A-containing concentrate prepared from cheese whey culture supernatant of Lactobacillus gasseri LA39', Animal Science Journal, vol. 84, no. 2, pp. 144-149. https://doi.org/10.1111/j.1740-0929.2012.01048.x
Nakamura, Kiyoshi ; Arakawa, Kensuke ; Kawai, Yasushi ; Yasuta, Narimi ; Chujo, Takahiro ; Watanabe, Masamichi ; Iioka, Hiroyuki ; Tanioka, Masashi ; Nishimura, Junko ; Kitazawa, Haruki ; Tsurumi, Koichi ; Saito, Tadao. / Food preservative potential of gassericin A-containing concentrate prepared from cheese whey culture supernatant of Lactobacillus gasseri LA39. In: Animal Science Journal. 2013 ; Vol. 84, No. 2. pp. 144-149.
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