Food deprivation induces monocarboxylate transporter 2 expression in the brainstem of female rat

Shuichi Matsuyama, Satoshi Ohkura, Kinuyo Iwata, Yoshihisa Uenoyama, Hiroko Tsukamura, Kei Ichiro Maeda, Koji Kimura

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Ketone bodies are considered to act as a signal to suppress gonadotropin release during negative energy balance. The present study examined the effect of 48-h fasting on the mRNA expressions of monocarboxylate transporter 1 (MCT1) and MCT2, which are involved in ketone body transport, in several brain regions. Quantitative real-time RT-PCR analysis showed that the MCT2 mRNA levels were significantly increased by 48-h fasting in the area postrema-solitary tract nucleus (AP-NTS) region but not the arcuate nucleus-ventromedial hypothalamic nucleus (ARC-VMH) and central gray-supragenual nucleus around the 4th ventricle (CG-SGe) regions. Fasting did not significantly affect MCT1 mRNA expression in any of the brain areas examined. Luteinizing hormone (LH) pulse frequency significantly decreased and plasma concentrations of β-hydroxybutyric acid, a ketone body, significantly increased after 48-h fasting. The present results suggest that increased uptake of ketone bodies via MCT2 in the AP-NTS region is likely involved in the mechanism of fasting-induced suppression of LH secretion in rats.

Original languageEnglish
Pages (from-to)256-261
Number of pages6
JournalJournal of Reproduction and Development
Volume55
Issue number3
DOIs
Publication statusPublished - Aug 10 2009
Externally publishedYes

Keywords

  • Fasting
  • Ketone body
  • Luteinizing hormone
  • Monocarboxylate transporter

ASJC Scopus subject areas

  • Animal Science and Zoology

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