TY - JOUR
T1 - Fluorescent bioprobes for visualization of puromycin-sensitive aminopeptidase in living cells
AU - Kakuta, Hiroki
AU - Koiso, Yukiko
AU - Nagasawa, Kazuo
AU - Hashimoto, Yuichi
N1 - Funding Information:
The work described in this paper was partially supported by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan. The authors are grateful to Dr. Akimitsu, Mr. Miyagi, and Mr. Ogawa (Olympus Co. Ltd.) for their technical support in obtaining fluorescence microscopic photographs.
PY - 2003/1/6
Y1 - 2003/1/6
N2 - Non-peptide, small-molecular, non-competitive inhibitors of puromycin-sensitive aminopeptidase (PSA), that is, 3-(2,6-diethylphenyl)-2,4(1H,3H)-quinazolinedione (PAQ-22, 3) and its 1N-methyl analogue (MPAQ-22 4), were structurally modified to afford fluorescent bioprobes, ANTAQ (5) and DAMPAQ-22 (6). The cellular localization of PSA could be visualized by the use of these fluorescent bioprobes.
AB - Non-peptide, small-molecular, non-competitive inhibitors of puromycin-sensitive aminopeptidase (PSA), that is, 3-(2,6-diethylphenyl)-2,4(1H,3H)-quinazolinedione (PAQ-22, 3) and its 1N-methyl analogue (MPAQ-22 4), were structurally modified to afford fluorescent bioprobes, ANTAQ (5) and DAMPAQ-22 (6). The cellular localization of PSA could be visualized by the use of these fluorescent bioprobes.
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U2 - 10.1016/S0960-894X(02)00845-4
DO - 10.1016/S0960-894X(02)00845-4
M3 - Article
C2 - 12467622
AN - SCOPUS:0037421050
SN - 0960-894X
VL - 13
SP - 83
EP - 86
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
IS - 1
ER -