Fluorescence property of photosystem II protein complexes bound to a gold nanoparticle

Kazuki Tahara, Ahmed Mohamed, Kousuke Kawahara, Ryo Nagao, Yuki Kato, Hiroshi Fukumura, Yutaka Shibata, Takumi Noguchi

Research output: Contribution to journalArticle

Abstract

Development of an efficient photo-anode system for water oxidation is key to the success of artificial photosynthesis. We previously assembled photosystem II (PSII) proteins, which are an efficient natural photocatalyst for water oxidation, on a gold nanoparticle (GNP) to prepare a PSII-GNP conjugate as an anode system in a light-driven water-splitting nano-device (Noji et al., J. Phys. Chem. Lett., 2011, 2, 2448-2452). In the current study, we characterized the fluorescence property of the PSII-GNP conjugate by static and time-resolved fluorescence measurements, and compared with that of free PSII proteins. It was shown that in a static fluorescence spectrum measured at 77 K, the amplitude of a major peak at 683 nm was significantly reduced and a red shoulder at 693 nm disappeared in PSII-GNP. Time-resolved fluorescence measurements showed that picosecond components at 683 nm decayed faster by factors of 1.4-2.1 in PSII-GNP than in free PSII, explaining the observed quenching of the major fluorescence peak. In addition, a nanosecond-decay component arising from a 'red chlorophyll' at 693 nm was lost in time-resolved fluorescence of PSII-GNP, probably due to a structural perturbation of this chlorophyll by interaction with GNP. Consistently with these fluorescence properties, degradation of PSII during strong-light illumination was two times slower in PSII-GNP than in free PSII. The enhanced durability of PSII is an advantageous property of the PSII-GNP conjugate in the development of an artificial photosynthesis device.

Original languageEnglish
Pages (from-to)121-134
Number of pages14
JournalFaraday Discussions
Volume198
DOIs
Publication statusPublished - 2017
Externally publishedYes

Fingerprint

Photosystem II Protein Complex
Gold
Nanoparticles
Fluorescence
gold
proteins
fluorescence
nanoparticles
photosynthesis
chlorophylls
Photosynthesis
anodes
Chlorophyll
oxidation
Water
water splitting
Anodes
Electrodes
shoulders
durability

ASJC Scopus subject areas

  • Medicine(all)
  • Physical and Theoretical Chemistry

Cite this

Tahara, K., Mohamed, A., Kawahara, K., Nagao, R., Kato, Y., Fukumura, H., ... Noguchi, T. (2017). Fluorescence property of photosystem II protein complexes bound to a gold nanoparticle. Faraday Discussions, 198, 121-134. https://doi.org/10.1039/c6fd00188b

Fluorescence property of photosystem II protein complexes bound to a gold nanoparticle. / Tahara, Kazuki; Mohamed, Ahmed; Kawahara, Kousuke; Nagao, Ryo; Kato, Yuki; Fukumura, Hiroshi; Shibata, Yutaka; Noguchi, Takumi.

In: Faraday Discussions, Vol. 198, 2017, p. 121-134.

Research output: Contribution to journalArticle

Tahara, K, Mohamed, A, Kawahara, K, Nagao, R, Kato, Y, Fukumura, H, Shibata, Y & Noguchi, T 2017, 'Fluorescence property of photosystem II protein complexes bound to a gold nanoparticle', Faraday Discussions, vol. 198, pp. 121-134. https://doi.org/10.1039/c6fd00188b
Tahara, Kazuki ; Mohamed, Ahmed ; Kawahara, Kousuke ; Nagao, Ryo ; Kato, Yuki ; Fukumura, Hiroshi ; Shibata, Yutaka ; Noguchi, Takumi. / Fluorescence property of photosystem II protein complexes bound to a gold nanoparticle. In: Faraday Discussions. 2017 ; Vol. 198. pp. 121-134.
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