Fluorescence lifetime probes for detection of RNA degradation

Riku Hirata, Kazutaka Hirakawa, Naotaka Shimada, Kazunori Watanabe, Takashi Ohtsuki

Research output: Contribution to journalArticlepeer-review

Abstract

To investigate RNA degradation in live cells, detection methods that do not require RNA extraction from cells are necessary. In this study, we examined the utility of fluorescence lifetime measurements using a probe attached to the end of an RNA molecule for detecting RNA degradation. We optimized a short fluorescein-labeled RNA sequence whose fluorescence lifetime varied significantly before and after degradation. The selected HHG-fluorescein sequence (H = U, C, or A) is a promising RNA labeling unit (fluorescence lifetime probe) for live cell imaging of RNA degradation.

Original languageEnglish
Pages (from-to)277-282
Number of pages6
JournalAnalyst
Volume146
Issue number1
DOIs
Publication statusPublished - Jan 7 2021

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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