TY - JOUR
T1 - Fluorescence in situ hybridization for molecular cytogenetic analysis in filamentous fungi.
AU - Tsuchiya, Dai
AU - Taga, Masatoki
PY - 2010
Y1 - 2010
N2 - Fluorescence in situ hybridization (FISH) is a powerful technology for studying eukaryotic chromosomes and genomes from the combined view of cytogenetics and molecular biology, but its use in filamentous fungi has been limited. In this chapter, we describe protocols to perform three basic FISH techniques in filamentous fungi: (a) FISH mapping of unique sequences on the somatic chromosomes and interphase nuclei, (b) chromosome painting to detect a specific chromosome in the genome by fluorescent painting of the whole chromosome, and (c) fiber FISH on the stretched DNA fibers for physical mapping. The ways of preparing target specimens unique to filamentous fungi are included in the protocols.
AB - Fluorescence in situ hybridization (FISH) is a powerful technology for studying eukaryotic chromosomes and genomes from the combined view of cytogenetics and molecular biology, but its use in filamentous fungi has been limited. In this chapter, we describe protocols to perform three basic FISH techniques in filamentous fungi: (a) FISH mapping of unique sequences on the somatic chromosomes and interphase nuclei, (b) chromosome painting to detect a specific chromosome in the genome by fluorescent painting of the whole chromosome, and (c) fiber FISH on the stretched DNA fibers for physical mapping. The ways of preparing target specimens unique to filamentous fungi are included in the protocols.
UR - http://www.scopus.com/inward/record.url?scp=77953640690&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77953640690&partnerID=8YFLogxK
U2 - 10.1007/978-1-60761-611-5_18
DO - 10.1007/978-1-60761-611-5_18
M3 - Article
C2 - 20238274
AN - SCOPUS:77953640690
SN - 1064-3745
VL - 638
SP - 235
EP - 257
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -