Fluorescence-guided surgery of retroperitoneal-implanted human fibrosarcoma in nude mice delays or eliminates tumor recurrence and increases survival compared to bright-light surgery

Fuminari Uehara, Yukihiko Hiroshima, Shinji Miwa, Yasunori Tome, Shuya Yano, Mako Yamamoto, Yasunori Matsumoto, Hiroki Maehara, Kazuhiro Tanaka, Michael Bouvet, Fuminori Kanaya, Robert M. Hoffman

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The aim of this study is to determine if fluorescence-guided surgery (FGS) can eradicate human fibrosarcoma growing in the retroperitoneum of nude mice. One week after retroperitoneal implantation of human HT1080 fibrosarcoma cells, expressing green fluorescent protein (GFP) (HT-1080-GFP), in nudemice, bright-light surgery (BLS) was performed on all tumor-bearing mice (n = 22). After BLS,mice were randomized into 2 treatment groups; BLS-only (n = 11) or the combination of BLS + FGS (n = 11). The residual tumors remaining after BLS were resected with FGS using a hand-held portable imaging systemunder fluorescence navigation. The average residual tumor area after BLS + FGS was significantly smaller than after BLS-only (0.4 ± 0.4 mm2 and 10.5 ± 2.4 mm2, respectively; p = 0.006). Five weeks after surgery, the fluorescent-tumor areas of BLS- and BLS + FGS-treated mice were 379 ± 147 mm2and 11.7 ± 6.9 mm2, respectively, indicating that FGS greatly inhibited tumor recurrence compared to BLS. The combination of BLS + FGS significantly decreased fibrosarcoma recurrence compared to BLS-only treated mice (p < 0.001). Mice treated with BLS+FGS had a significantly higher disease-free survival rate than mice treated with BLS-only at five weeks after surgery. These results suggest that combination of BLS + FGS significantly reduced the residual fibrosarcoma volume after BLS and improved disease-free survival.

Original languageEnglish
Article numbere0116865
JournalPloS one
Volume10
Issue number2
DOIs
Publication statusPublished - Feb 24 2015

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Fingerprint Dive into the research topics of 'Fluorescence-guided surgery of retroperitoneal-implanted human fibrosarcoma in nude mice delays or eliminates tumor recurrence and increases survival compared to bright-light surgery'. Together they form a unique fingerprint.

  • Cite this

    Uehara, F., Hiroshima, Y., Miwa, S., Tome, Y., Yano, S., Yamamoto, M., Matsumoto, Y., Maehara, H., Tanaka, K., Bouvet, M., Kanaya, F., & Hoffman, R. M. (2015). Fluorescence-guided surgery of retroperitoneal-implanted human fibrosarcoma in nude mice delays or eliminates tumor recurrence and increases survival compared to bright-light surgery. PloS one, 10(2), [e0116865]. https://doi.org/10.1371/journal.pone.0116865