Fluorescence-guided surgery improves outcome in an orthotopic osteosarcoma nude-mouse model

Shinji Miwa, Yukihiko Hiroshima, Shuya Yano, Yong Zhang, Yasunori Matsumoto, Fuminari Uehara, Mako Yamamoto, Hiroaki Kimura, Katsuhiro Hayashi, Michael Bouvet, Hiroyuki Tsuchiya, Robert M. Hoffman

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)


In order to develop a model for fluorescence-guided surgery (FGS), 143B human osteosarcoma cells expressing red fluorescent protein (RFP) were injected into the intramedullary cavity of the tibia in nude mice. The fluorescent areas of residual tumors after bright-light surgery (BLS) and FGS were 10.2±2.4mm2 and 0.1±0.1mm2, respectively (p<0.001). The BLS-treated mice and BLScisplatinum (CDDP)-treated mice had significant recurrence. In contrast, the FGS mice and FGSCDDP mice had very little recurring tumor growth. Disease-free survival (DFS) in the BLS-, BLSCDDP-, FGS-, and FGSCDDP-treated mice was 12.5%, 37.5%, 75.0%, and 87.5%, respectively. The FGS-treated mice had a significantly higher DFS rate than the BLS-treated mice (p=0.021). The FGSCDDP-treated mice had significantly higher DFS rate than the BLSCDDP-treated mice (p=0.043). Although chemotherapy significantly reduced multiple metastases (p=0.033), there was no significant correlation between FGS and lung metastasis. FGS significantly reduced the recurrence of the primary tumor but did not reduce lung metastasis. The combination of FGS and adjuvant CDDP reduced tumor recurrence and prevented multiple metastases. FGS and adjuvant chemotherapy should be performed as early as possible in the disease to prevent both recurrence and metastatic development.

Original languageEnglish
Pages (from-to)1596-1601
Number of pages6
JournalJournal of Orthopaedic Research
Issue number12
Publication statusPublished - Dec 1 2014
Externally publishedYes


  • Chemotherapy
  • Cisplatinum (CDDP)
  • Fluorescence-guided surgery
  • Orthotopic mouse model
  • Osteosarcoma
  • RFP

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine


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