Flow injection analysis of hydrogen peroxide using glass-beads modified with manganese(III)-tetra(4-carboxyphenyl)porphine derivative and its analytical application to the determination of serum glucose

Masaki Mifune, Katsuyoshi Sugimoto, Akimasa Iwado, Hiromichi Akizawa, Noriko Motohashi, Yutaka Saito

Research output: Contribution to journalArticle

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Abstract

A flow injection analysis system of hydrogen peroxide was developed. The present system is based on measuring of the absorbance of a quinoid dye formed by the following reaction catalyzed by peroxidase: Phenol + 4-Aminoantipyrine + 2H2O2Peroxidase Quinoid dye + 4H2O. A column packed with aminopropyl-glass beads modified with a manganese(III)-tetra(4-carboxyphenyl)porphine derivative (Mn-TCPPG column), which has peroxidase-like activity, was used in place of an immobilized peroxidase column in the above reaction. The linear range of the calibration curve was 0.4-80 μg/ml hydrogen peroxide. The relative standard deviation of this system was 2.97% (n = 100, 10 μg/ml hydrogen peroxide 20 μl injection). The Mn-TCPPG column has sufficiently stability for the continuous injection of hydrogen peroxide untill 100 times. The advantageous feature of the Mn-TCPPG column was a less-electrostatic interaction between the mother glass beads and the anionic chromogen or quinoid dye formed and the stability in terms of the storage, temperature and moisture. The determination of serum glucose was achieved by attaching an immobilized glucose oxidase column to this system without deproteinization.

Original languageEnglish
Pages (from-to)569-573
Number of pages5
JournalAnalytical Sciences
Volume19
Issue number4
DOIs
Publication statusPublished - Apr 1 2003

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Hydrogen Peroxide
Peroxidase
Derivatives
Glucose
Glass
Coloring Agents
Ampyrone
Glucose Oxidase
Phenol
Coulomb interactions
Moisture
Calibration
manganese(III)-tetra(4-carboxyphenyl)porphine
Temperature

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Flow injection analysis of hydrogen peroxide using glass-beads modified with manganese(III)-tetra(4-carboxyphenyl)porphine derivative and its analytical application to the determination of serum glucose. / Mifune, Masaki; Sugimoto, Katsuyoshi; Iwado, Akimasa; Akizawa, Hiromichi; Motohashi, Noriko; Saito, Yutaka.

In: Analytical Sciences, Vol. 19, No. 4, 01.04.2003, p. 569-573.

Research output: Contribution to journalArticle

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