Abstract
To investigate one of practical applications of the supports modified with metal-porphines as artificial solid-catalysts, columns into which the supports were packed were supplied to catalytic columns for a flow injection analysis (FIA) system for determination of ascorbic acid (AsA) by the following reactions:AsA+O2→dehydoroAsA+H2O2,H 2O2+chromogen→2H2O+Dye.Among the columns tested, the column containing silica gels modified with Co-tetrakis(carboxyphenyl)porphine catalyzed most rapidly the oxidation reaction of AsA that is accompanied by the formation of hydrogen peroxide. The resulting hydrogen peroxide was determined by FIA system equipped with the column containing glass beads modified with Mn-tetrakis(carboxyphenyl)porphine, which gave a linear calibration curve and large peak-areas of the range corresponding to AsA concentration between 0.2 and 10 μmol/ml. The results indicated that some supports modified with metal-porphine would be applicable to the FIA for AsA as the solid catalyses which function as if the immobilized enzymes.
| Original language | English |
|---|---|
| Pages (from-to) | 1923-1928 |
| Number of pages | 6 |
| Journal | Journal of Pharmaceutical and Biomedical Analysis |
| Volume | 30 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jan 15 2003 |
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Keywords
- Ascorbic acid
- Catalysis
- Flow injection analysis
- Metal-porphine
- Mimesis of enzyme
- Modified carriers
ASJC Scopus subject areas
- Analytical Chemistry
- Pharmaceutical Science
Cite this
Flow injection analysis of ascorbic acid using carriers modified with metal-porphine as oxidative solid catalyses. / Iwado, Akimasa; Mifune, Masaki; Akizawa, Hiromichi; Motohashi, Noriko; Saito, Yutaka.
In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 30, No. 6, 15.01.2003, p. 1923-1928.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Flow injection analysis of ascorbic acid using carriers modified with metal-porphine as oxidative solid catalyses
AU - Iwado, Akimasa
AU - Mifune, Masaki
AU - Akizawa, Hiromichi
AU - Motohashi, Noriko
AU - Saito, Yutaka
PY - 2003/1/15
Y1 - 2003/1/15
N2 - To investigate one of practical applications of the supports modified with metal-porphines as artificial solid-catalysts, columns into which the supports were packed were supplied to catalytic columns for a flow injection analysis (FIA) system for determination of ascorbic acid (AsA) by the following reactions:AsA+O2→dehydoroAsA+H2O2,H 2O2+chromogen→2H2O+Dye.Among the columns tested, the column containing silica gels modified with Co-tetrakis(carboxyphenyl)porphine catalyzed most rapidly the oxidation reaction of AsA that is accompanied by the formation of hydrogen peroxide. The resulting hydrogen peroxide was determined by FIA system equipped with the column containing glass beads modified with Mn-tetrakis(carboxyphenyl)porphine, which gave a linear calibration curve and large peak-areas of the range corresponding to AsA concentration between 0.2 and 10 μmol/ml. The results indicated that some supports modified with metal-porphine would be applicable to the FIA for AsA as the solid catalyses which function as if the immobilized enzymes.
AB - To investigate one of practical applications of the supports modified with metal-porphines as artificial solid-catalysts, columns into which the supports were packed were supplied to catalytic columns for a flow injection analysis (FIA) system for determination of ascorbic acid (AsA) by the following reactions:AsA+O2→dehydoroAsA+H2O2,H 2O2+chromogen→2H2O+Dye.Among the columns tested, the column containing silica gels modified with Co-tetrakis(carboxyphenyl)porphine catalyzed most rapidly the oxidation reaction of AsA that is accompanied by the formation of hydrogen peroxide. The resulting hydrogen peroxide was determined by FIA system equipped with the column containing glass beads modified with Mn-tetrakis(carboxyphenyl)porphine, which gave a linear calibration curve and large peak-areas of the range corresponding to AsA concentration between 0.2 and 10 μmol/ml. The results indicated that some supports modified with metal-porphine would be applicable to the FIA for AsA as the solid catalyses which function as if the immobilized enzymes.
KW - Ascorbic acid
KW - Catalysis
KW - Flow injection analysis
KW - Metal-porphine
KW - Mimesis of enzyme
KW - Modified carriers
UR - http://www.scopus.com/inward/record.url?scp=0037437703&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037437703&partnerID=8YFLogxK
U2 - 10.1016/S0731-7085(02)00536-8
DO - 10.1016/S0731-7085(02)00536-8
M3 - Article
C2 - 12485735
AN - SCOPUS:0037437703
VL - 30
SP - 1923
EP - 1928
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
SN - 0731-7085
IS - 6
ER -