Floral organ-specific and constitutive expression of an Arabidopsis thaliana heat-shock HSP18.2:: GUS fusion gene is retained even after homeotic conversion of flowers by mutation

Hirokazu Tsukaya, Taku Takahashi, Satoshi Naito, Yoshihumi Komeda

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Organ-specific and constitutive expression of the Arabidosis HSP18.2 gene under normal growth conditions (22° C) was observed in transgenic A. thaliana, which carried a fusion gene composed of the promoter region of HSP18.2, one of the genes for low molecular weight heat-shock proteins in Arabidopsis, and the gene for β-glucuronidase (GUS) from Escherichia coli. In order to clarify the organ-specific nature of promoter expression, various mutations that affect flower morphology were introduced into the transgenic Arabidopsis line, AHS9. The results show that the pattern of expression observed in sepals, filaments, and styles is regulated in a structure-dependent manner, and suggest that the HSP18.2 gene might have an important role in the process of differentiation of flower buds, as do several other stress-related genes.

Original languageEnglish
Pages (from-to)26-32
Number of pages7
JournalMGG Molecular & General Genetics
Volume237
Issue number1-2
DOIs
Publication statusPublished - Feb 1993
Externally publishedYes

Fingerprint

Gene Fusion
Glucuronidase
Arabidopsis
Shock
Hot Temperature
Mutation
Genes
Heat-Shock Proteins
Genetic Promoter Regions
Molecular Weight
Escherichia coli
Growth

Keywords

  • Arabidopsis thaliana
  • Floral organ
  • HSP

ASJC Scopus subject areas

  • Genetics

Cite this

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abstract = "Organ-specific and constitutive expression of the Arabidosis HSP18.2 gene under normal growth conditions (22° C) was observed in transgenic A. thaliana, which carried a fusion gene composed of the promoter region of HSP18.2, one of the genes for low molecular weight heat-shock proteins in Arabidopsis, and the gene for β-glucuronidase (GUS) from Escherichia coli. In order to clarify the organ-specific nature of promoter expression, various mutations that affect flower morphology were introduced into the transgenic Arabidopsis line, AHS9. The results show that the pattern of expression observed in sepals, filaments, and styles is regulated in a structure-dependent manner, and suggest that the HSP18.2 gene might have an important role in the process of differentiation of flower buds, as do several other stress-related genes.",
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T1 - Floral organ-specific and constitutive expression of an Arabidopsis thaliana heat-shock HSP18.2:: GUS fusion gene is retained even after homeotic conversion of flowers by mutation

AU - Tsukaya, Hirokazu

AU - Takahashi, Taku

AU - Naito, Satoshi

AU - Komeda, Yoshihumi

PY - 1993/2

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AB - Organ-specific and constitutive expression of the Arabidosis HSP18.2 gene under normal growth conditions (22° C) was observed in transgenic A. thaliana, which carried a fusion gene composed of the promoter region of HSP18.2, one of the genes for low molecular weight heat-shock proteins in Arabidopsis, and the gene for β-glucuronidase (GUS) from Escherichia coli. In order to clarify the organ-specific nature of promoter expression, various mutations that affect flower morphology were introduced into the transgenic Arabidopsis line, AHS9. The results show that the pattern of expression observed in sepals, filaments, and styles is regulated in a structure-dependent manner, and suggest that the HSP18.2 gene might have an important role in the process of differentiation of flower buds, as do several other stress-related genes.

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