Floral chromosomes of Arabidopsis thaliana for detecting low-copy DNA sequences by fluorescence in situ hybridization

M. Murata, F. Motoyoshi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Cosmid and plasmid clones containing 11 kb, or more, of genomic DNA sequences were mapped with high efficiencies using fluorescence in situ hybridization (FISH) to mitotic metaphase chromosomes prepared from floral tissues of Arabidopsis thaliana. The chromosomal locations were correlated with the map positions determined by RFLP (restriction fragment length polymorphism) analyses. Almost no signals were detected on the chromosomes of root meristematic tissues when FISH was performed with the same clones as probes. This discrepancy in efficiency of detection is possibly caused by the differences in chromatin structure between the root meristematic tissues and the floral tissues.

Original languageEnglish
Pages (from-to)39-43
Number of pages5
JournalChromosoma
Volume104
Issue number1
DOIs
Publication statusPublished - 1995

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Fluorescence In Situ Hybridization
Arabidopsis
Chromosomes
Clone Cells
Cosmids
Metaphase
Restriction Fragment Length Polymorphisms
Chromatin
Plasmids

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Floral chromosomes of Arabidopsis thaliana for detecting low-copy DNA sequences by fluorescence in situ hybridization. / Murata, M.; Motoyoshi, F.

In: Chromosoma, Vol. 104, No. 1, 1995, p. 39-43.

Research output: Contribution to journalArticle

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