Flexibility and strictness in functional replacement of domain III of cry insecticidal proteins from Bacillus thuringiensis

Hiroshi Sakai, Mohammad Tofazzal Hossain Howlader, Yumiko Ishida, Akiko Nakaguchi, Keiko Oka, Kouji Ohbayashi, Masashi Yamagiwa, Tohru Hayakawa

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Cry1C, one of the lepidopteran-specific insecticidal proteins from Bacillus thuringiensis, exhibits potent cytotoxicity against Sf9, an insect cell line. Cry1Aa and Cry4A, which are lepidopteran- and dipteran-specific insecticidal proteins, respectively, show no cytotoxicity against Sf9. When domain III of Cry1C was replaced with that of Cry1Aa or Cry4A, the hybrid Cry1C protein retained the cytotoxicity. These results suggest that domain III of Cry1C is not crucial in determining the cytocidal specificity of Cry1C against Sf9.

Original languageEnglish
Pages (from-to)381-383
Number of pages3
JournalJournal of Bioscience and Bioengineering
Volume103
Issue number4
DOIs
Publication statusPublished - Apr 2007

Fingerprint

Bacillus thuringiensis
Bacilli
Cytotoxicity
Proteins
Sf9 Cells
Insects
Cells
Cell Line

Keywords

  • Bacillus thuringiensis
  • Cry protein
  • cytotoxicity
  • domain exchange
  • Sf9

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Flexibility and strictness in functional replacement of domain III of cry insecticidal proteins from Bacillus thuringiensis. / Sakai, Hiroshi; Howlader, Mohammad Tofazzal Hossain; Ishida, Yumiko; Nakaguchi, Akiko; Oka, Keiko; Ohbayashi, Kouji; Yamagiwa, Masashi; Hayakawa, Tohru.

In: Journal of Bioscience and Bioengineering, Vol. 103, No. 4, 04.2007, p. 381-383.

Research output: Contribution to journalArticle

Sakai, Hiroshi ; Howlader, Mohammad Tofazzal Hossain ; Ishida, Yumiko ; Nakaguchi, Akiko ; Oka, Keiko ; Ohbayashi, Kouji ; Yamagiwa, Masashi ; Hayakawa, Tohru. / Flexibility and strictness in functional replacement of domain III of cry insecticidal proteins from Bacillus thuringiensis. In: Journal of Bioscience and Bioengineering. 2007 ; Vol. 103, No. 4. pp. 381-383.
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