Flavonol-containing phosphorylated pullulan may attenuate pulp inflammation

J. Yonehiro, Y. Yoshida, A. Yamashita, S. Yoshizawa, K. Ohta, N. Kamata, T. Okihara, F. Nishimura

Research output: Contribution to journalArticle

6 Citations (Scopus)


Aim: To find possible reagents to minimize inflammatory responses by using an established pulpitis models for the purpose of developing new pulp-capping materials, and to test the possible use of phosphorylated pullulan as a carrier for such an anti-inflammatory reagent. Methodology: Co-culturing was performed using transwell systems. Inflammatory responses were evaluated by measuring cytokines produced by the cells. The effects of two flavonoids, luteolin and quercetin, as anti-inflammatory reagents, and phosphorylated pullulan, which potentially achieves a sufficient marginal sealing to hydroxyapatite and slowly releases luteolin, as a carrier for flavonoids, were tested. Results: Flavonols, particularly luteolin, dramatically attenuated inflammatory cytokine production, which was augmented by co-cultures. Luteolin was successfully enclosed by phosphorylated pullulan. Finally, it was confirmed that luteolin released from phosphorylated pullulan was effective in reducing cytokine production by co-cultures. Conclusions: Combination of phosphorylated pullulan and luteolin could be potentially used in the treatment of dental pulp inflammation.

Original languageEnglish
Pages (from-to)119-127
Number of pages9
JournalInternational Endodontic Journal
Issue number2
Publication statusPublished - Feb 1 2013


  • Anti-inflammation
  • DP-1 pulp cells
  • Dental pulpitis
  • Flavonoids
  • Mesenchymal pulp cells
  • Phosphorylated pullulan
  • THP-1 macrophages

ASJC Scopus subject areas

  • Dentistry(all)

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    Yonehiro, J., Yoshida, Y., Yamashita, A., Yoshizawa, S., Ohta, K., Kamata, N., Okihara, T., & Nishimura, F. (2013). Flavonol-containing phosphorylated pullulan may attenuate pulp inflammation. International Endodontic Journal, 46(2), 119-127. https://doi.org/10.1111/j.1365-2591.2012.02095.x