Flagellin Glycosylation Island in Pseudomonas syringae pv. glycinea and Its Role in Host Specificity

Kasumi Takeuchi, Fumiko Taguchi, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

The deduced amino acid sequences of the flagellins of Pseudomonas syringae pv. tabaci and P. syringae pv. glycinea are identical; however, their abilities to induce a hypersensitive reaction are clearly different. The reason for the difference seems to depend on the posttranslational modification of the flagellins. To investigate the role of this posttranslational modification in the interactions between plants and bacterial pathogens, we isolated genes that are potentially involved in the posttranslational modification of flagellin in P. syringae pv. glycinea (glycosylation island); then defective mutants with mutations in these genes were generated. There are three open reading frames in the glycosylation island, designated orf1, orf2, and orf3. orf1 and orf2 encode putative glycosyltransferases, and mutants with defects in these open reading frames, Δorf1 and Δorf2, secreted nonglycosylated and slightly glycosylated flagellins, respectively. Inoculation tests performed with these mutants and original nonhost tobacco leaves revealed that Δorf1 and Δorj2 could grow on tobacco leaves and caused symptom-like changes. In contrast, these mutants failed to cause symptoms on original host soybean leaves. These data indicate that putative glycosyltransferases encoded in the flagellin glycosylation island are strongly involved in recognition by plants and could be the specific determinants of compatibility between phytopathogenic bacteria and plant species.

Original languageEnglish
Pages (from-to)6658-6665
Number of pages8
JournalJournal of Bacteriology
Volume185
Issue number22
DOIs
Publication statusPublished - Nov 2003

Fingerprint

Pseudomonas syringae
Flagellin
Host Specificity
Glycosylation
Islands
Post Translational Protein Processing
Glycosyltransferases
Open Reading Frames
Tobacco
Soybeans
Genes
Amino Acid Sequence
Bacteria
Mutation

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Flagellin Glycosylation Island in Pseudomonas syringae pv. glycinea and Its Role in Host Specificity. / Takeuchi, Kasumi; Taguchi, Fumiko; Inagaki, Yoshishige; Toyoda, Kazuhiro; Shiraishi, Tomonori; Ichinose, Yuki.

In: Journal of Bacteriology, Vol. 185, No. 22, 11.2003, p. 6658-6665.

Research output: Contribution to journalArticle

Takeuchi, Kasumi ; Taguchi, Fumiko ; Inagaki, Yoshishige ; Toyoda, Kazuhiro ; Shiraishi, Tomonori ; Ichinose, Yuki. / Flagellin Glycosylation Island in Pseudomonas syringae pv. glycinea and Its Role in Host Specificity. In: Journal of Bacteriology. 2003 ; Vol. 185, No. 22. pp. 6658-6665.
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