Flagellin glycosylation is ubiquitous in a broad range of phytopathogenic bacteria

Yuki Ichinose, Fumiko Taguchi, Masanobu Yamamoto, Mayumi Ohnishi-Kameyama, Tatsuo Atsumi, Masako Iwaki, Hiromi Manabe, Mio Kumagai, Quan Thanh Nguyen, Chi Linh Nguyen, Yoshishige Inagaki, Hiroshi Ono, Kazuhiro Chiku, Tadashi Ishii, Mitsuru Yoshida

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Glycosylation of flagellin is known to be involved in filament stabilization, motility, and virulence in Pseudomonas syringae. Here we investigated flagellin glycosylation in other phytopathogenic bacteria. Analyses of deduced amino acid sequences, glycostaining, and molecular masses of purified flagellins revealed that flagellins from all phytopathogenic bacteria investigated were glycosylated. Furthermore, the flagellin in a glycosylation-defective mutant of Xanthomonas campestris pv. campestris (Xcc) had a reduced molecular mass, and motility and virulence of the mutant toward host leaves decreased. These results suggest that flagellin glycosylation is ubiquitous in most phytopathogenic bacteria and that flagellin glycosylation is required for virulence in Xcc.

Original languageEnglish
Pages (from-to)359-365
Number of pages7
JournalJournal of General Plant Pathology
Volume79
Issue number5
DOIs
Publication statusPublished - Sep 2013

Fingerprint

flagellin
plant pathogenic bacteria
glycosylation
Xanthomonas campestris pv. campestris
virulence
molecular weight
mutants
Pseudomonas syringae
amino acid sequences

Keywords

  • Flagellin
  • Glycosylation
  • Motility
  • Virulence

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Ichinose, Y., Taguchi, F., Yamamoto, M., Ohnishi-Kameyama, M., Atsumi, T., Iwaki, M., ... Yoshida, M. (2013). Flagellin glycosylation is ubiquitous in a broad range of phytopathogenic bacteria. Journal of General Plant Pathology, 79(5), 359-365. https://doi.org/10.1007/s10327-013-0464-4

Flagellin glycosylation is ubiquitous in a broad range of phytopathogenic bacteria. / Ichinose, Yuki; Taguchi, Fumiko; Yamamoto, Masanobu; Ohnishi-Kameyama, Mayumi; Atsumi, Tatsuo; Iwaki, Masako; Manabe, Hiromi; Kumagai, Mio; Nguyen, Quan Thanh; Nguyen, Chi Linh; Inagaki, Yoshishige; Ono, Hiroshi; Chiku, Kazuhiro; Ishii, Tadashi; Yoshida, Mitsuru.

In: Journal of General Plant Pathology, Vol. 79, No. 5, 09.2013, p. 359-365.

Research output: Contribution to journalArticle

Ichinose, Y, Taguchi, F, Yamamoto, M, Ohnishi-Kameyama, M, Atsumi, T, Iwaki, M, Manabe, H, Kumagai, M, Nguyen, QT, Nguyen, CL, Inagaki, Y, Ono, H, Chiku, K, Ishii, T & Yoshida, M 2013, 'Flagellin glycosylation is ubiquitous in a broad range of phytopathogenic bacteria', Journal of General Plant Pathology, vol. 79, no. 5, pp. 359-365. https://doi.org/10.1007/s10327-013-0464-4
Ichinose, Yuki ; Taguchi, Fumiko ; Yamamoto, Masanobu ; Ohnishi-Kameyama, Mayumi ; Atsumi, Tatsuo ; Iwaki, Masako ; Manabe, Hiromi ; Kumagai, Mio ; Nguyen, Quan Thanh ; Nguyen, Chi Linh ; Inagaki, Yoshishige ; Ono, Hiroshi ; Chiku, Kazuhiro ; Ishii, Tadashi ; Yoshida, Mitsuru. / Flagellin glycosylation is ubiquitous in a broad range of phytopathogenic bacteria. In: Journal of General Plant Pathology. 2013 ; Vol. 79, No. 5. pp. 359-365.
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