Fission of tubular endosomes triggers endosomal acidification and movement

Kumi Mesaki, Kenji Tanabe, Masanori Obayashi, Natsuko Oe, Kohji Takei

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The early endosome acts as a sorting station for internalized molecules destined for recycling or degradation. While recycled molecules are sorted and delivered to tubular endosomes, residual compartments containing molecules to be degraded undergo "maturation" before final degradation in the lysosome. This maturation involves acidification, microtubule-dependent motility, and perinuclear localization. It is currently unknown how sorting and the processes of maturation cooperate with each other. Here, we show that fission of a tubular endosome triggers the maturation of the residual endosome, leading to degradation. Use of the dynamin inhibitor dynasore to block tubular endosome fission inhibited acidification, endosomal motility along microtubules, perinuclear localization, and degradation. However, tubular endosome fission was not affected by inhibiting endosomal acidification or by depolymerizing the microtubules. These results demonstrate that the fission of recycling tubules is the first important step in endosomal maturation and degradation in the lysosome. We believe this to be the first evidence of a cascade from sorting to degradation.

Original languageEnglish
Article numbere19764
JournalPLoS One
Volume6
Issue number5
DOIs
Publication statusPublished - 2011

Fingerprint

endosomes
Acidification
Endosomes
acidification
Degradation
degradation
Sorting
Microtubules
sorting
microtubules
Recycling
lysosomes
Lysosomes
Molecules
recycling
Dynamins
Corrosion inhibitors

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Fission of tubular endosomes triggers endosomal acidification and movement. / Mesaki, Kumi; Tanabe, Kenji; Obayashi, Masanori; Oe, Natsuko; Takei, Kohji.

In: PLoS One, Vol. 6, No. 5, e19764, 2011.

Research output: Contribution to journalArticle

Mesaki, Kumi ; Tanabe, Kenji ; Obayashi, Masanori ; Oe, Natsuko ; Takei, Kohji. / Fission of tubular endosomes triggers endosomal acidification and movement. In: PLoS One. 2011 ; Vol. 6, No. 5.
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