First evidence for occurrence of galβ1-3GlcNAcβ1-4man unit in N-glycans of insect glycoprotein: β1-3gal and β1-4GlcNAc transferases are involved in N-glycan processing of royal jelly glycoproteins

Yoshinobu Kimura, Kazunori Tsumura, Mariko Kimura, Kiyoshi Okihara, Hiroyuki Sugimoto, Hideo Yamada

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

On a way of structural analysis of total N-glycans linked to glycoproteins in royal jelly (Kimura, Y. et al., Biosci. Biotechnol. Biochem., 64, 2109-2120 (2000), Kimura, M. et al., Biosci. Biotechnol. Biochem., 66, 1985-1989 (2002)), we found that some complex type N-glycans containing a β1-3galactose residue occur on the insect glycoproteins. Up to date, it has been considered that naturally occurring insect glycoproteins do not bear the galactose-containing N-glycans, therefore, in this report we describe the structural analysis of the complex type N-glycans of royal jelly glycoproteins. By a combination of endo- and exo-glycosidase digestions, IS-MS analysis, and 1H-NMR spectroscopy, the structures of the β1-3 galactose-containing N-glycan were identified as the following; GlcNAcβ1-2Manα1-6[GlcNAcβ1-2(Galβ1-3GlcNAcβ1-4)Manα1-3]Manβ1-4GlcNAcβ1-4GlcNAc, Manα1-3Manα1-6[GlcNAcβ1-2(Galβ1-3GlcNAcβ1-4)Manα1-3]Manβ1-4GlcNAcβ1-4GlcNAc, and Manα1-6(Manα1-3)Manα1-6[GlcNAcβ1-2(Galβ1-3GlcNAcβ1-4)Manα1-3]Manβ1-4GlcNAcβ1-4GlcNAc. To our knowledge, this is the first report showing that the Galβ1-3GlcNAcβ1-4Man unit occurs in N-glycans of insect glycoproteins, indicating a β1-3 galactosyl transferase and β1-4GlcNAc transferase (GNT-IV) are expressed in the honeybee cells.

Original languageEnglish
Pages (from-to)1852-1856
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume67
Issue number8
DOIs
Publication statusPublished - Jan 1 2003

Keywords

  • Apis mellifera
  • Insect glycoprotein
  • N-glycan
  • Royal jelly
  • β1-3galactosyl transferase

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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