First evidence for occurrence of Galβ1-3GlcNAcβ1-4Man unit in N-glycans of insect glycoprotein: β1-3Gal and β1-4GlcNAc transferases are involved in N-glycan processing of royal jelly glycoproteins

Yoshinobu Kimura, Kazunori Tsumura, Mariko Kimura, Kiyoshi Okihara, Hiroyuki Sugimoto, Hideo Yamada

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

On a way of structural analysis of total N-glycans linked to glycoproteins in royal jelly (Kimura, Y. et al., Biosci. Biotechnol. Biochem., 64, 2109-2120 (2000), Kimura, M. et al., Biosci. Biotechnol. Biochem., 66, 1985-1989 (2002)), we found that some complex type N-glycans containing a β1-3galactose residue occur on the insect glycoproteins. Up to date, it has been considered that naturally occurring insect glycoproteins do not bear the galactose-containing N-glycans, therefore, in this report we describe the structural analysis of the complex type N-glycans of royal jelly glycoproteins. By a combination of endo- and exo-glycosidase digestions, IS-MS analysis, and 1H-NMR spectroscopy, the structures of the β1-3 galactose-containing N-glycan were identified as the following; GlcNAcβ1-2Manα1-6[GlcNAcβ1-2(Galβ1-3GlcNAcβ1-4) Manα1-3]Manβ1-4GlcNAcβ1-4GlcNAc, Manα1-3Manα1- 6[GlcNAcβ1-2(Galβ1-3GlcNAcβ1-4)Manα1-3]Manβ1- 4GlcNAcβ1-4GlcNAc, and Manα1-6(Manα1-3)Manα1- 6[GlcNAcβ1-2(Galβ1-3GlcNAcβ1-4)Manα1-3]Manβ1- 4GlcNAcβ1-4GlcNAc. To our knowledge, this is the first report showing that the Galβ1-3GlcNAcβ1-4Man unit occurs in N-glycans of insect glycoproteins, indicating a β1-3 galactosyl transferase and β1-4GlcNAc transferase (GNT-IV) are expressed in the honeybee cells.

Original languageEnglish
Pages (from-to)1852-1856
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume67
Issue number8
Publication statusPublished - Aug 2003

Fingerprint

royal jelly
Glycoproteins
Transferases
transferases
Polysaccharides
Insects
glycoproteins
polysaccharides
insects
Processing
Structural analysis
glycosidases
Glycoside Hydrolases
Galactose
galactose
Nuclear magnetic resonance spectroscopy
honey bees
nuclear magnetic resonance spectroscopy
Digestion
Magnetic Resonance Spectroscopy

Keywords

  • β1-3galactosyl transferase
  • Apis mellifera
  • Insect glycoprotein
  • N-glycan
  • Royal jelly

ASJC Scopus subject areas

  • Food Science
  • Applied Microbiology and Biotechnology
  • Chemistry (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering

Cite this

First evidence for occurrence of Galβ1-3GlcNAcβ1-4Man unit in N-glycans of insect glycoprotein : β1-3Gal and β1-4GlcNAc transferases are involved in N-glycan processing of royal jelly glycoproteins. / Kimura, Yoshinobu; Tsumura, Kazunori; Kimura, Mariko; Okihara, Kiyoshi; Sugimoto, Hiroyuki; Yamada, Hideo.

In: Bioscience, Biotechnology and Biochemistry, Vol. 67, No. 8, 08.2003, p. 1852-1856.

Research output: Contribution to journalArticle

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abstract = "On a way of structural analysis of total N-glycans linked to glycoproteins in royal jelly (Kimura, Y. et al., Biosci. Biotechnol. Biochem., 64, 2109-2120 (2000), Kimura, M. et al., Biosci. Biotechnol. Biochem., 66, 1985-1989 (2002)), we found that some complex type N-glycans containing a β1-3galactose residue occur on the insect glycoproteins. Up to date, it has been considered that naturally occurring insect glycoproteins do not bear the galactose-containing N-glycans, therefore, in this report we describe the structural analysis of the complex type N-glycans of royal jelly glycoproteins. By a combination of endo- and exo-glycosidase digestions, IS-MS analysis, and 1H-NMR spectroscopy, the structures of the β1-3 galactose-containing N-glycan were identified as the following; GlcNAcβ1-2Manα1-6[GlcNAcβ1-2(Galβ1-3GlcNAcβ1-4) Manα1-3]Manβ1-4GlcNAcβ1-4GlcNAc, Manα1-3Manα1- 6[GlcNAcβ1-2(Galβ1-3GlcNAcβ1-4)Manα1-3]Manβ1- 4GlcNAcβ1-4GlcNAc, and Manα1-6(Manα1-3)Manα1- 6[GlcNAcβ1-2(Galβ1-3GlcNAcβ1-4)Manα1-3]Manβ1- 4GlcNAcβ1-4GlcNAc. To our knowledge, this is the first report showing that the Galβ1-3GlcNAcβ1-4Man unit occurs in N-glycans of insect glycoproteins, indicating a β1-3 galactosyl transferase and β1-4GlcNAc transferase (GNT-IV) are expressed in the honeybee cells.",
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AU - Kimura, Yoshinobu

AU - Tsumura, Kazunori

AU - Kimura, Mariko

AU - Okihara, Kiyoshi

AU - Sugimoto, Hiroyuki

AU - Yamada, Hideo

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AB - On a way of structural analysis of total N-glycans linked to glycoproteins in royal jelly (Kimura, Y. et al., Biosci. Biotechnol. Biochem., 64, 2109-2120 (2000), Kimura, M. et al., Biosci. Biotechnol. Biochem., 66, 1985-1989 (2002)), we found that some complex type N-glycans containing a β1-3galactose residue occur on the insect glycoproteins. Up to date, it has been considered that naturally occurring insect glycoproteins do not bear the galactose-containing N-glycans, therefore, in this report we describe the structural analysis of the complex type N-glycans of royal jelly glycoproteins. By a combination of endo- and exo-glycosidase digestions, IS-MS analysis, and 1H-NMR spectroscopy, the structures of the β1-3 galactose-containing N-glycan were identified as the following; GlcNAcβ1-2Manα1-6[GlcNAcβ1-2(Galβ1-3GlcNAcβ1-4) Manα1-3]Manβ1-4GlcNAcβ1-4GlcNAc, Manα1-3Manα1- 6[GlcNAcβ1-2(Galβ1-3GlcNAcβ1-4)Manα1-3]Manβ1- 4GlcNAcβ1-4GlcNAc, and Manα1-6(Manα1-3)Manα1- 6[GlcNAcβ1-2(Galβ1-3GlcNAcβ1-4)Manα1-3]Manβ1- 4GlcNAcβ1-4GlcNAc. To our knowledge, this is the first report showing that the Galβ1-3GlcNAcβ1-4Man unit occurs in N-glycans of insect glycoproteins, indicating a β1-3 galactosyl transferase and β1-4GlcNAc transferase (GNT-IV) are expressed in the honeybee cells.

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KW - Apis mellifera

KW - Insect glycoprotein

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KW - Royal jelly

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