First cleavage of enucleated rat eggs following transplantation of karyoplast removed from pronuclear eggs stored at 2 to 6°C for various durations

K. Niwa, Hiroaki Funahashi, K. Ohmae, M. Kattoh

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2 Citations (Scopus)

Abstract

Pronuclear rat eggs were cultured for 24 to 48 hours at 37°C after storage at 2 to 6°C for 0 to 216 hours in medium. Very high proportions (89 to 97%) of eggs cleaved to the two-cell stage after storage for 0 to 48 hours. The proportion, however, decreased rapidly in eggs stored for 72 hours (60%), and eggs stored for more than 120 hours cleaved poorly. However, when male and female pronuclei from stored eggs were transplanted into enucleated fresh eggs, 92 to 100% of the fused eggs with karyoplast stored for 0 to 144 hours cleaved. Although the cleavage rate was reduced to 50% when karyoplast from eggs stored for 168 hours was transplanted, this reduction was not significant. Complete loss of cleaving ability was observed in fused eggs with the karyoplast stored for 216 hours. These results clearly indicate that the pronuclei of rat eggs can be stored for a longer period than the cytoplasm at low temperatures (2 to 6°C).

Original languageEnglish
Pages (from-to)411-417
Number of pages7
JournalTheriogenology
Volume36
Issue number3
DOIs
Publication statusPublished - 1991

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Keywords

  • low temperature storage
  • nuclear transplantation
  • pronuclear egg
  • rat

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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