Fine mapping of quantitative trait loci Hd-1, Hd-2 and Hd-3, controlling heading date of rice, as single Mendelian factors

Toshio Yamamoto, Y. Kuboki, S. Y. Lin, T. Sasaki, M. Yano

Research output: Contribution to journalArticle

185 Citations (Scopus)

Abstract

Fine mapping was carried out on three putative QTLs (tentatively designated as Hd-1 to Hd-3) of five such QTLs controlling heading date in rice that had been earlier identified using an F2 population derived from a cross between a japonica variety, 'Nipponbare', and an indica variety, 'Kasalath', using progeny backcrossed with 'Nipponbare' as the recurrent parent. One BC3F2 and two BC3F1 plants, in which the target QTL regions were heterozygous and most other chromosomal regions were homozygous for the 'Nipponbare' allele, were selected as the experimental material. Self-pollinated progeny (BC3F2 and BC3F3) of the BC3F1 or BC3F2 showed continuous variation in days to heading. By means of progeny testing based on BC3F3 or BC3F4 lines, we determined the genotypes of each BC3F2 or BC3F3 individual at target QTLs. Their segregation patterns fitted Mendelian inheritance ratios. When the results obtained by RFLP analysis and progeny tests were combined, Hd-1, Hd-2 and Hd-3 were mapped precisely on chromosomes 6, 7 and 6, respectively, of a rice RFLP linkage map. The results demonstrated that QTLs can be treated as Mendelian factors. Moreover, these precise locations were in good agreement with the regions estimated by QTL analysis of the initial F2 population, demonstrating the high reliability of QTL mapping using a high-density linkage map.

Original languageEnglish
Pages (from-to)37-44
Number of pages8
JournalTheoretical And Applied Genetics
Volume97
Issue number1-2
DOIs
Publication statusPublished - Jul 1 1998
Externally publishedYes

Fingerprint

Quantitative Trait Loci
heading
Restriction Fragment Length Polymorphisms
quantitative trait loci
rice
Chromosomes, Human, Pair 6
Chromosomes, Human, Pair 7
Population
Alleles
Genotype
chromosome mapping
restriction fragment length polymorphism
Mendelian inheritance
progeny testing
Oryza
alleles
chromosomes
genotype

Keywords

  • Backcross progeny
  • Heading date
  • Oryza sativa L.
  • QTL
  • RFLP mapping

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

Cite this

Fine mapping of quantitative trait loci Hd-1, Hd-2 and Hd-3, controlling heading date of rice, as single Mendelian factors. / Yamamoto, Toshio; Kuboki, Y.; Lin, S. Y.; Sasaki, T.; Yano, M.

In: Theoretical And Applied Genetics, Vol. 97, No. 1-2, 01.07.1998, p. 37-44.

Research output: Contribution to journalArticle

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