Fine mapping of Carbon assimilation rate 8, a quantitative trait locus for flag leaf nitrogen content, stomatal conductance and photosynthesis in rice

Shunsuke Adachi, Kazuaki Yoshikawa, Utako Yamanouchi, Takanari Tanabata, Jian Sun, Taiichiro Ookawa, Toshio Yamamoto, Rowan F. Sage, Tadashi Hirasawa, Junichi Yonemaru

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Increasing the rate of leaf photosynthesis is one important approach for increasing grain yield in rice (Oryza sativa). Exploiting the natural variation in CO2 assimilation rate (A) between rice cultivars using quantitative genetics is one promising means to identify genes contributing to higher photosynthesis. In this study, we determinedprecise location of Carbon Assimilation Rate 8 (CAR8) by crossing a high-yielding indica cultivar with a Japanese commercial cultivar. Fine mapping suggested that CAR8 encodes a putative Heme Activator Protein 3 (OsHAP3) subunit of a CCAAT-box-binding transcription factor called OsHAP3H. Sequencing analysis revealed that the indica allele of CAR8 has a 1-bp deletion at 322 bp from the start codon, resulting in a truncated protein of 125 amino acids. In addition, CAR8 is identical to DTH8/Ghd8/LHD1, which was reported to control rice flowering date. The increase of A is largely due to an increase of RuBP regeneration rate via increased leaf nitrogen content, and partially explained by reduced stomatal limitation via increased stomatal conductance relative to A. This allele also increases hydraulic conductivity, which would promote higher stomatal conductance. This indicates that CAR8 affects multiple physiological aspects relating to photosynthesis. The detailed analysis of molecular functions of CAR8 would help to understand the association between photosynthesis and flowering and demonstrate specific genetic mechanisms that can be exploited to improve photosynthesis in rice and potentially other crops.

Original languageEnglish
Article number60
JournalFrontiers in Plant Science
Volume8
Issue numberJANUARY
DOIs
Publication statusPublished - Jan 31 2017
Externally publishedYes

Fingerprint

nitrogen content
stomatal conductance
quantitative trait loci
photosynthesis
rice
leaves
cultivars
flowering
alleles
start codon
quantitative genetics
heme
hydraulic conductivity
carbon dioxide fixation
assimilation (physiology)
Oryza sativa
transcription factors
proteins
grain yield
carbon dioxide

Keywords

  • Leaf nitrogen content
  • Oryza sativa
  • Photosynthesis
  • Quantitative trait locus
  • RuBP regeneration
  • Stomatal conductance

ASJC Scopus subject areas

  • Plant Science

Cite this

Fine mapping of Carbon assimilation rate 8, a quantitative trait locus for flag leaf nitrogen content, stomatal conductance and photosynthesis in rice. / Adachi, Shunsuke; Yoshikawa, Kazuaki; Yamanouchi, Utako; Tanabata, Takanari; Sun, Jian; Ookawa, Taiichiro; Yamamoto, Toshio; Sage, Rowan F.; Hirasawa, Tadashi; Yonemaru, Junichi.

In: Frontiers in Plant Science, Vol. 8, No. JANUARY, 60, 31.01.2017.

Research output: Contribution to journalArticle

Adachi, Shunsuke ; Yoshikawa, Kazuaki ; Yamanouchi, Utako ; Tanabata, Takanari ; Sun, Jian ; Ookawa, Taiichiro ; Yamamoto, Toshio ; Sage, Rowan F. ; Hirasawa, Tadashi ; Yonemaru, Junichi. / Fine mapping of Carbon assimilation rate 8, a quantitative trait locus for flag leaf nitrogen content, stomatal conductance and photosynthesis in rice. In: Frontiers in Plant Science. 2017 ; Vol. 8, No. JANUARY.
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