Fine genetic mapping of the nuclear gene, Rf-1, that restores the BT-type cytoplasmic male sterility in rice (Oryza sativa L.) by PCR-based markers

Toshiyuki Komori, Toshio Yamamoto, Naoki Takemori, Masakazu Kashihara, Hideko Matsushima, Naoto Nitta

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The Rf-1 gene restores the pollen fertility disturbed by the BT-type male sterile cytoplasm. Nine restriction fragment length polymorphism (RFLP) markers on chromosome 10, where the Rf-1 gene is located, were converted to PCR-based markers. Genetic analysis was conducted using the nine markers and a segregating population consisting of 1042 plants. As a result, the Rf-1 gene was mapped between S12564 Tsp509I and C1361 MwoI. The genetic distance of the two marker loci was estimated as 0.3 cM. Based on the map information of the Rf-1 gene, it will be possible to introduce a limited chromosomal segment of the Rf-1 region derived from a donor into japonica rice varieties efficiently and effectively by marker-assisted selection (MAS). Also, examination of seed purity of hybrid rice varieties and their parental lines will be facilitated. Furthermore, judging from the genetic distance from the Rf-1 locus to S12564 Tsp509I and C1361 MwoI loci, it seems that the Rf-1 gene could be isolated by chromosomal walking from either or both of the two marker loci.

Original languageEnglish
Pages (from-to)241-247
Number of pages7
JournalEuphytica
Volume129
Issue number2
DOIs
Publication statusPublished - Feb 19 2003
Externally publishedYes

Keywords

  • Cytoplasmic male sterility
  • DNA marker
  • Fertility restorer gene
  • Hybrid rice
  • Marker-assisted selection
  • Rf-1

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science
  • Horticulture

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