Fine genetic mapping of the nuclear gene, Rf-1, that restores the BT-type cytoplasmic male sterility in rice (Oryza sativa L.) by PCR-based markers

Toshiyuki Komori, Toshio Yamamoto, Naoki Takemori, Masakazu Kashihara, Hideko Matsushima, Naoto Nitta

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The Rf-1 gene restores the pollen fertility disturbed by the BT-type male sterile cytoplasm. Nine restriction fragment length polymorphism (RFLP) markers on chromosome 10, where the Rf-1 gene is located, were converted to PCR-based markers. Genetic analysis was conducted using the nine markers and a segregating population consisting of 1042 plants. As a result, the Rf-1 gene was mapped between S12564 Tsp509I and C1361 MwoI. The genetic distance of the two marker loci was estimated as 0.3 cM. Based on the map information of the Rf-1 gene, it will be possible to introduce a limited chromosomal segment of the Rf-1 region derived from a donor into japonica rice varieties efficiently and effectively by marker-assisted selection (MAS). Also, examination of seed purity of hybrid rice varieties and their parental lines will be facilitated. Furthermore, judging from the genetic distance from the Rf-1 locus to S12564 Tsp509I and C1361 MwoI loci, it seems that the Rf-1 gene could be isolated by chromosomal walking from either or both of the two marker loci.

Original languageEnglish
Pages (from-to)241-247
Number of pages7
JournalEuphytica
Volume129
Issue number2
DOIs
Publication statusPublished - Feb 19 2003
Externally publishedYes

Fingerprint

cytoplasmic male sterility
Chromosome Mapping
Male Infertility
chromosome mapping
Oryza sativa
rice
Polymerase Chain Reaction
loci
Genes
genes
genetic distance
Chromosomes, Human, Pair 10
Pollen
marker-assisted selection
Restriction Fragment Length Polymorphisms
purity
walking
Walking
Fertility
genetic techniques and protocols

Keywords

  • Cytoplasmic male sterility
  • DNA marker
  • Fertility restorer gene
  • Hybrid rice
  • Marker-assisted selection
  • Rf-1

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Horticulture
  • Plant Science

Cite this

Fine genetic mapping of the nuclear gene, Rf-1, that restores the BT-type cytoplasmic male sterility in rice (Oryza sativa L.) by PCR-based markers. / Komori, Toshiyuki; Yamamoto, Toshio; Takemori, Naoki; Kashihara, Masakazu; Matsushima, Hideko; Nitta, Naoto.

In: Euphytica, Vol. 129, No. 2, 19.02.2003, p. 241-247.

Research output: Contribution to journalArticle

Komori, Toshiyuki ; Yamamoto, Toshio ; Takemori, Naoki ; Kashihara, Masakazu ; Matsushima, Hideko ; Nitta, Naoto. / Fine genetic mapping of the nuclear gene, Rf-1, that restores the BT-type cytoplasmic male sterility in rice (Oryza sativa L.) by PCR-based markers. In: Euphytica. 2003 ; Vol. 129, No. 2. pp. 241-247.
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