Filament structure, organization, and dynamics in MreB sheets

David Popp, Akihiro Narita, Kayo Maeda, Tetsuro Fujisawa, Umesh Ghoshdastider, Mitsusada Iwasa, Yuichiro Maéda, Robert C. Robinson

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

In vivo fluorescence microscopy studies of bacterial cells have shown that the bacterial shape-determining protein and actin homolog, MreB, forms cable-like structures that spiral around the periphery of the cell. The molecular structure of these cables has yet to be established. Here we show by electron microscopy that Thermatoga maritime MreB forms complex, several μm long multilayered sheets consisting of diagonally interwoven filaments in the presence of either ATP or GTP. This architecture, in agreement with recent rheological measurements on MreB cables, may have superior mechanical properties and could be an important feature for maintaining bacterial cell shape. MreB polymers within the sheets appear to be single-stranded helical filaments rather than the linear protofilaments found in the MreB crystal structure. Sheet assembly occurs over a wide range of pH, ionic strength, and temperature. Polymerization kinetics are consistent with a cooperative assembly mechanism requiring only two steps: monomer activation followed by elongation. Steady-state TIRF microscopy studies of MreB suggest filament treadmilling while high pressure small angle x-ray scattering measurements indicate that the stability of MreB polymers is similar to that of F-actin filaments. In the presence of ADP or GDP, long, thin cables formed in which MreB was arranged in parallel as linear proto filaments. This suggests that the bacterial cell may exploit various nucleotides to generate different filament structures within cables for specific MreB-based functions.

Original languageEnglish
Pages (from-to)15858-15865
Number of pages8
JournalJournal of Biological Chemistry
Volume285
Issue number21
DOIs
Publication statusPublished - May 21 2010
Externally publishedYes

Fingerprint

Cables
Actins
Polymers
Cell Shape
Guanosine Triphosphate
Molecular Structure
Actin Cytoskeleton
Fluorescence Microscopy
Polymerization
Osmolar Concentration
Adenosine Diphosphate
Microscopy
Electron Microscopy
Nucleotides
Adenosine Triphosphate
X-Rays
Fluorescence microscopy
Pressure
Ionic strength
Temperature

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Popp, D., Narita, A., Maeda, K., Fujisawa, T., Ghoshdastider, U., Iwasa, M., ... Robinson, R. C. (2010). Filament structure, organization, and dynamics in MreB sheets. Journal of Biological Chemistry, 285(21), 15858-15865. https://doi.org/10.1074/jbc.M109.095901

Filament structure, organization, and dynamics in MreB sheets. / Popp, David; Narita, Akihiro; Maeda, Kayo; Fujisawa, Tetsuro; Ghoshdastider, Umesh; Iwasa, Mitsusada; Maéda, Yuichiro; Robinson, Robert C.

In: Journal of Biological Chemistry, Vol. 285, No. 21, 21.05.2010, p. 15858-15865.

Research output: Contribution to journalArticle

Popp, D, Narita, A, Maeda, K, Fujisawa, T, Ghoshdastider, U, Iwasa, M, Maéda, Y & Robinson, RC 2010, 'Filament structure, organization, and dynamics in MreB sheets', Journal of Biological Chemistry, vol. 285, no. 21, pp. 15858-15865. https://doi.org/10.1074/jbc.M109.095901
Popp D, Narita A, Maeda K, Fujisawa T, Ghoshdastider U, Iwasa M et al. Filament structure, organization, and dynamics in MreB sheets. Journal of Biological Chemistry. 2010 May 21;285(21):15858-15865. https://doi.org/10.1074/jbc.M109.095901
Popp, David ; Narita, Akihiro ; Maeda, Kayo ; Fujisawa, Tetsuro ; Ghoshdastider, Umesh ; Iwasa, Mitsusada ; Maéda, Yuichiro ; Robinson, Robert C. / Filament structure, organization, and dynamics in MreB sheets. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 21. pp. 15858-15865.
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