Fig mosaic emaravirus p4 protein is involved in cell-to-cell movement

Kazuya Ishikawa, Kensaku Maejima, Ken Komatsu, Osamu Netsu, Takuya Keima, Takuya Shiraishi, Yukari Okano, Masayoshi Hashimoto, Yasuyuki Yamaji, Shigetou Namba

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Fig mosaic virus (FMV), a member of the newly formed genus Emaravirus, is a segmented negative-strand RNA virus. Each of the six genomic FMV segments contains a single ORF: that of RNA4 encodes the protein p4. FMV-p4 is presumed to be the movement protein (MP) of the virus; however, direct experimental evidence for this is lacking. We assessed the intercellular distribution of FMV-p4 in plant cells by confocal laser scanning microscopy and we found that FMV-p4 was localized to plasmodesmata and to the plasma membrane accompanied by tubulelike structures. A series of experiments designed to examine the movement functions revealed that FMV-p4 has the capacity to complement viral cell-to-cell movement, prompt GFP diffusion between cells, and spread by itself to neighbouring cells. Altogether, our findings demonstrated that FMV-p4 shares several properties with other viral MPs and plays an important role in cell-tocell movement.

Original languageEnglish
Pages (from-to)682-686
Number of pages5
JournalJournal of General Virology
Volume94
Issue numberPART3
DOIs
Publication statusPublished - 2013
Externally publishedYes

ASJC Scopus subject areas

  • Virology

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