Fibulin-5 contributes to microfibril assembly in human periodontal ligament cells

Yutaka Hisanaga, Kazuki Nakashima, Eichi Tsuruga, Yuka Nakatomi, Yuji Hatakeyama, Hiroyuki Ishikawa, Yoshihiko Sawa

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The elastic system fibers comprise oxytalan, elaunin and elastic fibers, which differ in their relative microfibril and elastin content. Human periodontal ligaments (PDL) contain only oxytalan fibers (pure microfibrils) among them. Since fibulin-5 regulates the organization of elastic fibers to link the fibers to cells, we hypothesized that fibulin-5 may contribute to the formation of oxytalan fibers. We used siRNA for fibulin-5 in PDL cell culture to examine the extracellular deposition of fibrillin-1 and -2, which are the major components of microfibrils. Fibulin-5 was labeled on microfibrils positive for fibrillin-1 and -2. Fibulin-5 suppression reduced the level of fibrillin-1 and -2 deposition to 60% of the control level. These results suggest that fibulin-5 may control the formation of oxytalan fibers, and play a role in the homeostasis of oxytalan fibers.

Original languageEnglish
Pages (from-to)151-157
Number of pages7
JournalActa Histochemica et Cytochemica
Volume42
Issue number5
DOIs
Publication statusPublished - Oct 30 2009
Externally publishedYes

Fingerprint

Microfibrils
Periodontal Ligament
Ligaments
Elastic Tissue
Fibers
Elastin
Small Interfering RNA
fibulin
Level control
Homeostasis
Cell Culture Techniques
Cell culture
oxytalan
Fibrillin-1
Fibrillin-2

Keywords

  • Fibrillin
  • Fibulin-5
  • Microfibrils
  • Oxytalan fiber
  • Periodontal ligaments

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Physiology
  • Biochemistry
  • Cell Biology

Cite this

Hisanaga, Y., Nakashima, K., Tsuruga, E., Nakatomi, Y., Hatakeyama, Y., Ishikawa, H., & Sawa, Y. (2009). Fibulin-5 contributes to microfibril assembly in human periodontal ligament cells. Acta Histochemica et Cytochemica, 42(5), 151-157. https://doi.org/10.1267/ahc.09021

Fibulin-5 contributes to microfibril assembly in human periodontal ligament cells. / Hisanaga, Yutaka; Nakashima, Kazuki; Tsuruga, Eichi; Nakatomi, Yuka; Hatakeyama, Yuji; Ishikawa, Hiroyuki; Sawa, Yoshihiko.

In: Acta Histochemica et Cytochemica, Vol. 42, No. 5, 30.10.2009, p. 151-157.

Research output: Contribution to journalArticle

Hisanaga, Y, Nakashima, K, Tsuruga, E, Nakatomi, Y, Hatakeyama, Y, Ishikawa, H & Sawa, Y 2009, 'Fibulin-5 contributes to microfibril assembly in human periodontal ligament cells', Acta Histochemica et Cytochemica, vol. 42, no. 5, pp. 151-157. https://doi.org/10.1267/ahc.09021
Hisanaga Y, Nakashima K, Tsuruga E, Nakatomi Y, Hatakeyama Y, Ishikawa H et al. Fibulin-5 contributes to microfibril assembly in human periodontal ligament cells. Acta Histochemica et Cytochemica. 2009 Oct 30;42(5):151-157. https://doi.org/10.1267/ahc.09021
Hisanaga, Yutaka ; Nakashima, Kazuki ; Tsuruga, Eichi ; Nakatomi, Yuka ; Hatakeyama, Yuji ; Ishikawa, Hiroyuki ; Sawa, Yoshihiko. / Fibulin-5 contributes to microfibril assembly in human periodontal ligament cells. In: Acta Histochemica et Cytochemica. 2009 ; Vol. 42, No. 5. pp. 151-157.
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