Fibulin-4 and -5, but not Fibulin-2, are Associated with Tropoelastin Deposition in Elastin-Producing Cell Culture

Yoshinori Yamauchi, Eichi Tsuruga, Kazuki Nakashima, Yoshihiko Sawa, Hiroyuki Ishikawa

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

201E0l aTshteic J sapyasnte Smo cfiibeteyr so fc oHnisstioscth oefm misitcrryo afinbdri lCs ya-nd tropoelastin. During development, microfibrils act as a template on which tropoelastin is deposited. Fibrillin-1 is the major component of microfibrils. It is not clear whether elastic fiber-associated molecules, such as fibulins, contribute to tropoelastin deposition. Among the fibulin family, fibulin-2, -4 and -5 are capable of binding to tropoelastin and fibrillin-1. In the present study, we used the RNA interference (RNAi) technique to establish individual gene-specific knockdown of fibulin-2, -4 and -5 in elastin-producing cells (human gingival fibroblasts; HGF). We then examined the extracellular deposition of tropoelastin using immunofluorescence. RNAi-mediated downregulation of fibulin-4 and -5 was responsible for the diminution of tropoelastin deposition. Suppression of fibulin-5 appeared to inhibit the formation of fibrillin-1 microfibrils, while that of fibulin-4 did not. Similar results to those for HGF were obtained with human dermal fibroblasts. These results suggest that fibulin-4 and -5 may be associated in different ways with the extracellular deposition of tropoelastin during elastic fiber formation in elastinproducing cells in culture.

Original languageEnglish
Pages (from-to)131-138
Number of pages8
JournalACTA HISTOCHEMICA ET CYTOCHEMICA
Volume43
Issue number6
DOIs
Publication statusPublished - 2010
Externally publishedYes

Keywords

  • Elastic fiber
  • Fibrillin
  • Fibulin
  • Microfibrils
  • Tropoelastin

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biochemistry
  • Physiology
  • Histology
  • Cell Biology

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