TY - JOUR
T1 - Fibronectin type I repeat is a nonactivating ligand for EphA1 and inhibits ATF3-dependent angiogenesis
AU - Masuda, Junko
AU - Usui, Ryosuke
AU - Maru, Yoshiro
N1 - Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2008/5/9
Y1 - 2008/5/9
N2 - ATF3 stimulated promoter activity of EphA1 by 3.4-fold in ATF3-dependent angiogenesis in vitro. Although tyrosine kinase activation of EphA1 was dispensable, binding of EphA1 to fibronectin through its type I repeat played an essential role in the angiogenesis. Recombinant proteins containing fibronectin 10th to 12th type I repeat (I 10-12) but not I 12 could inhibit the angiogenesis in vitro by competitively targeting EphA1 with the full-length fibronectin. However, I 12 acquired a higher affinity toward EphA2 with K d 18 nM and inhibited vascular endothelial growth factor-dependent angiogenic invasion in a Matrigel plug assay.
AB - ATF3 stimulated promoter activity of EphA1 by 3.4-fold in ATF3-dependent angiogenesis in vitro. Although tyrosine kinase activation of EphA1 was dispensable, binding of EphA1 to fibronectin through its type I repeat played an essential role in the angiogenesis. Recombinant proteins containing fibronectin 10th to 12th type I repeat (I 10-12) but not I 12 could inhibit the angiogenesis in vitro by competitively targeting EphA1 with the full-length fibronectin. However, I 12 acquired a higher affinity toward EphA2 with K d 18 nM and inhibited vascular endothelial growth factor-dependent angiogenic invasion in a Matrigel plug assay.
UR - http://www.scopus.com/inward/record.url?scp=45149128636&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=45149128636&partnerID=8YFLogxK
U2 - 10.1074/jbc.M702164200
DO - 10.1074/jbc.M702164200
M3 - Article
C2 - 18308734
AN - SCOPUS:45149128636
VL - 283
SP - 13148
EP - 13155
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 19
ER -