Fibronectin-induced ductal formation in salivary gland self-organization model

Mahmoud Farahat, Gulsan A.S. Kazi, Hiroaki Taketa, Emilio S. Hara, Masamitsu Oshima, Takuo Kuboki, Takuya Matsumoto

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Recent advances in tissue regeneration approaches including 3D organoids, were based on various 3D organogenesis models. However, 3D models are generally technique-sensitive and time-consuming. Thus, we utilized an existing model of submandibular salivary gland (SMG) to modify a simple and highly reproducible in vitro 3D culture model of primary SMG cells self-organization into a well-developed cell spheroid inside Matrigel substrate. We used this model to observe the collective multicellular behavior during spheroid formation. Further, we applied various quantitative approaches including real-time live imaging and immune histochemical image analysis to dissect the cellular dynamics during tissue patterning. Results: On a time-scale of hours, we observed marked size and shape transformations in the developed 3D spheroid which resulted in a spatially-controlled growth differential from the canter to the periphery of the formed aggregates. Moreover, we investigated the effect of fibronectin (FN) on SMG cells self-organization using our simplified culture model. Interestingly, we discovered a novel role of FN in inducing duct-like elongation during initial stages of SMG bud formation. Conclusion: This in vitro model provides an excellent tool for analyzing the intercellular dynamics during early SMG tissue development as well as revealing a novel role of FN in SMG ductal expansion.

Original languageEnglish
Pages (from-to)813-825
Number of pages13
JournalDevelopmental Dynamics
Volume248
Issue number9
DOIs
Publication statusPublished - Sep 1 2019

Keywords

  • 3D organoids
  • ductal formation
  • extracellular matrix
  • fibronectin
  • salivary gland
  • self-organization

ASJC Scopus subject areas

  • Developmental Biology

Fingerprint Dive into the research topics of 'Fibronectin-induced ductal formation in salivary gland self-organization model'. Together they form a unique fingerprint.

  • Cite this