Fibronectin-induced ductal formation in salivary gland self-organization model

Mahmoud Farahat, Gulsan A.S. Kazi, Hiroaki Taketa, Emilio satoshi Hara, Masamitsu Oshima, Takuo Kuboki, Takuya Matsumoto

Research output: Contribution to journalArticle

Abstract

Background: Recent advances in tissue regeneration approaches including 3D organoids, were based on various 3D organogenesis models. However, 3D models are generally technique-sensitive and time-consuming. Thus, we utilized an existing model of submandibular salivary gland (SMG) to modify a simple and highly reproducible in vitro 3D culture model of primary SMG cells self-organization into a well-developed cell spheroid inside Matrigel substrate. We used this model to observe the collective multicellular behavior during spheroid formation. Further, we applied various quantitative approaches including real-time live imaging and immune histochemical image analysis to dissect the cellular dynamics during tissue patterning. Results: On a time-scale of hours, we observed marked size and shape transformations in the developed 3D spheroid which resulted in a spatially-controlled growth differential from the canter to the periphery of the formed aggregates. Moreover, we investigated the effect of fibronectin (FN) on SMG cells self-organization using our simplified culture model. Interestingly, we discovered a novel role of FN in inducing duct-like elongation during initial stages of SMG bud formation. Conclusion: This in vitro model provides an excellent tool for analyzing the intercellular dynamics during early SMG tissue development as well as revealing a novel role of FN in SMG ductal expansion.

Original languageEnglish
JournalDevelopmental Dynamics
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Submandibular Gland
Salivary Glands
Fibronectins
Organoids
Organogenesis
Regeneration
Growth

Keywords

  • 3D organoids
  • ductal formation
  • extracellular matrix
  • fibronectin
  • salivary gland
  • self-organization

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Fibronectin-induced ductal formation in salivary gland self-organization model. / Farahat, Mahmoud; Kazi, Gulsan A.S.; Taketa, Hiroaki; Hara, Emilio satoshi; Oshima, Masamitsu; Kuboki, Takuo; Matsumoto, Takuya.

In: Developmental Dynamics, 01.01.2019.

Research output: Contribution to journalArticle

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