Fibronectin-binding proteins secreted by mycobacterium avium

Hideki Kitaura, Naoya Oohara, Mariko Naito, Kazuhide Kobayashi, Takeshi Yamada

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Mycobacterium avium is an intracellular pathogen and a major opportunistic infectious agent observed in patients with acquired immune deficiency syndrome (AIDS). Fibronectin is an extracellular matrix protein and is a virulence factor for several extracellular pathogenic bacteria binding to mucosal surfaces. We investigated the fibronectin (FN)-binding proteins in the culture filtrate of M. avium by two-dimensional electrophoresis (2DE). Proteins in Sauton medium of M. avium after 3 weeks were separated by 2DE. The proteins were blotted onto polyvinylidene difluoride membrane and incubated with FN. FN-binding proteins were detected by Western blotting using anti-FN antibody. FN bound to five spots (33 kDa, 32 kDa, 31 kDa, 30 kDa and 25 kDa). N-terminal amino acids of these were determined. The 33 kDa spot corresponded to antigen 85 (Ag 85) C. The 32 and 31 kDa spots were either Ag 85 A or Ag 85 B. The 30 kDa spot corresponded to Ag 85 B of M. avium. The 25 kDa spot corresponded to MPA51 (M. avium MPB51). Thus, FN bound exclusively to the Ag 85 complex and MPA51.

Original languageEnglish
Pages (from-to)558-564
Number of pages7
JournalAPMIS
Volume108
Issue number9
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Mycobacterium avium
Fibronectins
Carrier Proteins
Antigens
Extracellular Matrix Proteins
Virulence Factors
Electrophoresis
Anti-Idiotypic Antibodies
Acquired Immunodeficiency Syndrome
Proteins
Western Blotting
Bacteria
Amino Acids
Membranes

Keywords

  • Antigen 85 complex
  • Fibronectin
  • Mycobacterium avium
  • Western blotting

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Microbiology (medical)
  • Immunology

Cite this

Kitaura, H., Oohara, N., Naito, M., Kobayashi, K., & Yamada, T. (2000). Fibronectin-binding proteins secreted by mycobacterium avium. APMIS, 108(9), 558-564.

Fibronectin-binding proteins secreted by mycobacterium avium. / Kitaura, Hideki; Oohara, Naoya; Naito, Mariko; Kobayashi, Kazuhide; Yamada, Takeshi.

In: APMIS, Vol. 108, No. 9, 2000, p. 558-564.

Research output: Contribution to journalArticle

Kitaura, H, Oohara, N, Naito, M, Kobayashi, K & Yamada, T 2000, 'Fibronectin-binding proteins secreted by mycobacterium avium', APMIS, vol. 108, no. 9, pp. 558-564.
Kitaura H, Oohara N, Naito M, Kobayashi K, Yamada T. Fibronectin-binding proteins secreted by mycobacterium avium. APMIS. 2000;108(9):558-564.
Kitaura, Hideki ; Oohara, Naoya ; Naito, Mariko ; Kobayashi, Kazuhide ; Yamada, Takeshi. / Fibronectin-binding proteins secreted by mycobacterium avium. In: APMIS. 2000 ; Vol. 108, No. 9. pp. 558-564.
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