Fibronectin Accelerates the Growth and Differentiation of Ameloblast Lineage Cells in Vitro

Makoto J. Tabata, Tatsushi Matsumura, Takafumi Fujii, Makoto Abe, Kojiro Kurisu

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

During tooth development, the growth and differentiation of ameloblast lineage (AL) cells are regulated by epithelial-mesenchymal interactions. To examine the dynamic effects of components of the basement membrane, which is the extracellular matrix (ECM) lying between the epithelium and mesenchyme, we prepared AL cells from the epithelial layer sheet of mandibular incisors of postnatal day 7 rats and cultured them on plates coated with type IV collagen, laminin-1, or fibronectin. The growth of AL cells was supported by type IV collagen and fibronectin but not by laminin-1 in comparison with that on type I collagen as a reference. Clustering and differentiation of AL cells were observed on all matrices examined. AL cells showed normal growth and differentiation at low cell density on fibronectin but not on type I collagen. Furthermore, the population of cytokeratin 14-positive cells on fibronectin was lower than that on other ECM components, suggesting that fibronectin may be a modulator to accelerate the differentiation of AL cells. After the cells had been cultured for 9 days on fibronectin, crystal-like structures were observed. These structures overlaid the cell clusters and were positive for von Kossa staining. These findings indicate that each matrix component has a regulative role in the proliferation and differentiation of AL cells and that fibronectin causes the greatest acceleration of AL cell differentiation.

Original languageEnglish
Pages (from-to)1673-1679
Number of pages7
JournalJournal of Histochemistry and Cytochemistry
Volume51
Issue number12
DOIs
Publication statusPublished - Dec 2003

Keywords

  • Ameloblast lineage cells
  • Crystal-like structures
  • Fibronectin
  • Laminin-1
  • Type I collagen
  • Type IV collagen

ASJC Scopus subject areas

  • Anatomy
  • Histology

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