Fibroblasts expressing Sonic hedgehog induce osteoblast differentiation and ectopic bone formation

Naoki Kinto, Masahiro Iwamoto, Motomi Enomoto-Iwamoto, Sumihare Noji, Hideyo Ohuchi, Hidefumi Yoshioka, Hiroko Kataoka, Yasuhiro Wada, Gao Yuhao, Hideaki E. Takahashi, Shusaku Yoshiki, Akira Yamaguchi

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

We investigated the role of Sonic hedgehog (SHH) in osteoblast differentiation and bone formation. The numbers of ALP-positive cells in the mouse fibroblastic cell line C3H10T1/2 and the mouse osteoblastic cell line MC3T3-E1 were increased by co-culture with chicken fibroblasts transfected with chicken Shh cDNA encoding amino-terminal peptide (Shh-N). The conditioned medium of Shh-N-RCAS-transfected chicken fibroblast cultures also significantly increased ALP activity in both C3H10T1/2 and MC3T3-E1 cells. Intramuscular transplantation of Shh-N-RCAS-transfected chicken fibroblasts into athymic mice induced ectopic bone formation. These results indicate that SHH induces osteoblast differentiation and ectopic bone formation.

Original languageEnglish
Pages (from-to)319-323
Number of pages5
JournalFEBS Letters
Volume404
Issue number2-3
DOIs
Publication statusPublished - Mar 10 1997
Externally publishedYes

Fingerprint

Hedgehogs
Osteoblasts
Fibroblasts
Osteogenesis
Chickens
Bone
Cells
Cell culture
Conditioned Culture Medium
Cell Line
Complementary DNA
Coculture Techniques
Nude Mice
Peptides
Transplantation

Keywords

  • Bone formation
  • Osteoblast differentiation
  • Sonic hedgehog

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Kinto, N., Iwamoto, M., Enomoto-Iwamoto, M., Noji, S., Ohuchi, H., Yoshioka, H., ... Yamaguchi, A. (1997). Fibroblasts expressing Sonic hedgehog induce osteoblast differentiation and ectopic bone formation. FEBS Letters, 404(2-3), 319-323. https://doi.org/10.1016/S0014-5793(97)00014-8

Fibroblasts expressing Sonic hedgehog induce osteoblast differentiation and ectopic bone formation. / Kinto, Naoki; Iwamoto, Masahiro; Enomoto-Iwamoto, Motomi; Noji, Sumihare; Ohuchi, Hideyo; Yoshioka, Hidefumi; Kataoka, Hiroko; Wada, Yasuhiro; Yuhao, Gao; Takahashi, Hideaki E.; Yoshiki, Shusaku; Yamaguchi, Akira.

In: FEBS Letters, Vol. 404, No. 2-3, 10.03.1997, p. 319-323.

Research output: Contribution to journalArticle

Kinto, N, Iwamoto, M, Enomoto-Iwamoto, M, Noji, S, Ohuchi, H, Yoshioka, H, Kataoka, H, Wada, Y, Yuhao, G, Takahashi, HE, Yoshiki, S & Yamaguchi, A 1997, 'Fibroblasts expressing Sonic hedgehog induce osteoblast differentiation and ectopic bone formation', FEBS Letters, vol. 404, no. 2-3, pp. 319-323. https://doi.org/10.1016/S0014-5793(97)00014-8
Kinto, Naoki ; Iwamoto, Masahiro ; Enomoto-Iwamoto, Motomi ; Noji, Sumihare ; Ohuchi, Hideyo ; Yoshioka, Hidefumi ; Kataoka, Hiroko ; Wada, Yasuhiro ; Yuhao, Gao ; Takahashi, Hideaki E. ; Yoshiki, Shusaku ; Yamaguchi, Akira. / Fibroblasts expressing Sonic hedgehog induce osteoblast differentiation and ectopic bone formation. In: FEBS Letters. 1997 ; Vol. 404, No. 2-3. pp. 319-323.
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