Fibrinogen adsorption on hydroxyapatite, carbonate apatite and gold surfaces in situ detected by quartz crystal microbalance with resistance technique

Hiroshi Yonekura, Motohiro Tagaya, Tomohiko Yoshioka, Toshiyuki Ikoma, Junzo Tanaka

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

When a biomaterial is implanted into the body, blood proteins adsorb on its surface and subsequently cells adhere via the protein adlayer. Thus, the understanding of protein adsorption and conformational change on the biomaterial surfaces is of great importance to control the biocompatibility such as antithrombotic properties and cell adhesion behaviors. In this study, we synthesized hydroxyapatite (HAp) and carbonate apatite (CAp) by a wet method. Then we successfully fabricated the HAp and CAp sensors for QCM-R by an electrophoretic deposition method. Adsorption behavior of proteins on the bone substitute material can be monitored by using these apatite sensors. Bovine serum albumin and fibrinogen were employed for the model proteins, and monitored the adsorption behavior on the HAp, CAp and reference gold (Au) sensors by the QCM-R technique. As a result, we revealed that fibrinogen and bovine serum albumin adsorbs on the gold surface by hydrophobic interaction, and adsorbs on the HAp and CAp surfaces mainly by electrostatic force. Besides, we revealed that fibrinogen adsorbs on the Au surface more rigid than on the HAp and CAp surfaces while bovine serum albumin adsorbs on the HAp and CAp surface more rigidly than on the Au surface.

Original languageEnglish
Title of host publicationGels and Biomedical Materials
Pages247-252
Number of pages6
DOIs
Publication statusPublished - Aug 20 2012
Externally publishedYes
Event2011 MRS Fall Meeting - Boston, MA, United States
Duration: Nov 28 2011Dec 2 2011

Publication series

NameMaterials Research Society Symposium Proceedings
Volume1418
ISSN (Print)0272-9172

Other

Other2011 MRS Fall Meeting
CountryUnited States
CityBoston, MA
Period11/28/1112/2/11

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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    Yonekura, H., Tagaya, M., Yoshioka, T., Ikoma, T., & Tanaka, J. (2012). Fibrinogen adsorption on hydroxyapatite, carbonate apatite and gold surfaces in situ detected by quartz crystal microbalance with resistance technique. In Gels and Biomedical Materials (pp. 247-252). (Materials Research Society Symposium Proceedings; Vol. 1418). https://doi.org/10.1557/opl.2012.397