FGF7 and FGF10 directly induce the apical ectodermal ridge in chick embryos

Sayuri Yonei-Tamura, Tetsuya Endo, Hiroshi Yajima, Hideyo Ohuchi, Hiroyuki Ide, Koji Tamura

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

During vertebrate limb development, the apical ectodermal ridge (AER) plays a vital role in both limb initiation and distal outgrowth of the limb bud. In the early chick embryo the prelimb bud mesoderm induces the AER in the overlying ectoderm. However, the direct inducer of the AER remains unknown. Here we report that FGF7 and FGF10, members of the fibroblast growth factor family, are the best candidates for the direct inducer of the AER. FGF7 induces an ectopic AER in the flank ectoderm of the chick embryo in a different manner from FGF1, -2, and -4 and activates the expression of Fgf8, an AER marker gene, in a cultured flank ectoderm without the mesoderm. Remarkably, FGF7 and FGF10 applied in the back induced an ectopic AER in the dorsal median ectoderm. Our results suggest that FGF7 and FGF10 directly induce the AER in the ectoderm both of the flank and of the dorsal midline and that these two regions have the competence for AER induction. Formation of the AER of the dorsal median ectoderm in the chick embryo is likely to appear as a vestige of the dorsal fin of the ancestors.

Original languageEnglish
Pages (from-to)133-143
Number of pages11
JournalDevelopmental Biology
Volume211
Issue number1
DOIs
Publication statusPublished - Jul 1 1999
Externally publishedYes

Fingerprint

Ectoderm
Chick Embryo
Mesoderm
Extremities
Limb Buds
Fibroblast Growth Factor 1
Fibroblast Growth Factors
Mental Competency
Vertebrates
Genes

Keywords

  • Apical ectodermal ridge (AER)
  • FGF10
  • FGF7
  • Limb morphogenesis

ASJC Scopus subject areas

  • Developmental Biology

Cite this

FGF7 and FGF10 directly induce the apical ectodermal ridge in chick embryos. / Yonei-Tamura, Sayuri; Endo, Tetsuya; Yajima, Hiroshi; Ohuchi, Hideyo; Ide, Hiroyuki; Tamura, Koji.

In: Developmental Biology, Vol. 211, No. 1, 01.07.1999, p. 133-143.

Research output: Contribution to journalArticle

Yonei-Tamura, S, Endo, T, Yajima, H, Ohuchi, H, Ide, H & Tamura, K 1999, 'FGF7 and FGF10 directly induce the apical ectodermal ridge in chick embryos', Developmental Biology, vol. 211, no. 1, pp. 133-143. https://doi.org/10.1006/dbio.1999.9290
Yonei-Tamura, Sayuri ; Endo, Tetsuya ; Yajima, Hiroshi ; Ohuchi, Hideyo ; Ide, Hiroyuki ; Tamura, Koji. / FGF7 and FGF10 directly induce the apical ectodermal ridge in chick embryos. In: Developmental Biology. 1999 ; Vol. 211, No. 1. pp. 133-143.
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