FGF10 maintains stem cell population during mouse incisor development

Hidemitsu Harada, Takashi Toyono, Kuniaki Toyoshima, Hideyo Ohuchi

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Mouse incisors have a cervical loop that gives rise to dental epithelium in the apical region of the tooth germ, in contrast to molars. In a study of formation of the stem-cell compartment, we focused on expression patterns of fibroblast growth factor (Fgf) 10 and Fgf3 in developing mice incisors. At E14, Fgf10 and Fgf3 were coexpressed in the dental papilla. After E16 mesenchymal cells underlying cervical loop expressed Fgf10 but not Fgf3. To illustrate the role of FGF10, we analyzed incisor development of Fgf10-deficient mice. The germs of FGF10-null mice proceeded to cap stage normally. However, at a later stage, the cervical loop was not formed. Functional disorder of FGF10 by a neutralizing anti-FGF10 antibody induced apoptosis in the cervical loop of incisor explants. Recombinant FGF10 rescued the cervical loop from apoptosis. These results show that FGF10 maintains the stem-cell compartment in the developing incisor tooth germ.

Original languageEnglish
Pages (from-to)201-204
Number of pages4
JournalConnective Tissue Research
Volume43
Issue number2-3
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Incisor
Stem cells
Stem Cells
Fibroblast Growth Factor 10
Apoptosis
Tooth Germ
Population
Dental Papilla
Antibodies
Neutralizing Antibodies
Anti-Idiotypic Antibodies
Tooth
Epithelium

Keywords

  • Epithelial-mesenchymal interactions
  • FGF10
  • Incisor development
  • Stem cells

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Nephrology
  • Cell Biology
  • Orthopedics and Sports Medicine

Cite this

FGF10 maintains stem cell population during mouse incisor development. / Harada, Hidemitsu; Toyono, Takashi; Toyoshima, Kuniaki; Ohuchi, Hideyo.

In: Connective Tissue Research, Vol. 43, No. 2-3, 2002, p. 201-204.

Research output: Contribution to journalArticle

Harada, H, Toyono, T, Toyoshima, K & Ohuchi, H 2002, 'FGF10 maintains stem cell population during mouse incisor development', Connective Tissue Research, vol. 43, no. 2-3, pp. 201-204.
Harada, Hidemitsu ; Toyono, Takashi ; Toyoshima, Kuniaki ; Ohuchi, Hideyo. / FGF10 maintains stem cell population during mouse incisor development. In: Connective Tissue Research. 2002 ; Vol. 43, No. 2-3. pp. 201-204.
@article{379c3bf7a5a34a7eb2767cb937d6efb9,
title = "FGF10 maintains stem cell population during mouse incisor development",
abstract = "Mouse incisors have a cervical loop that gives rise to dental epithelium in the apical region of the tooth germ, in contrast to molars. In a study of formation of the stem-cell compartment, we focused on expression patterns of fibroblast growth factor (Fgf) 10 and Fgf3 in developing mice incisors. At E14, Fgf10 and Fgf3 were coexpressed in the dental papilla. After E16 mesenchymal cells underlying cervical loop expressed Fgf10 but not Fgf3. To illustrate the role of FGF10, we analyzed incisor development of Fgf10-deficient mice. The germs of FGF10-null mice proceeded to cap stage normally. However, at a later stage, the cervical loop was not formed. Functional disorder of FGF10 by a neutralizing anti-FGF10 antibody induced apoptosis in the cervical loop of incisor explants. Recombinant FGF10 rescued the cervical loop from apoptosis. These results show that FGF10 maintains the stem-cell compartment in the developing incisor tooth germ.",
keywords = "Epithelial-mesenchymal interactions, FGF10, Incisor development, Stem cells",
author = "Hidemitsu Harada and Takashi Toyono and Kuniaki Toyoshima and Hideyo Ohuchi",
year = "2002",
language = "English",
volume = "43",
pages = "201--204",
journal = "Connective Tissue Research",
issn = "0300-8207",
publisher = "Informa Healthcare",
number = "2-3",

}

TY - JOUR

T1 - FGF10 maintains stem cell population during mouse incisor development

AU - Harada, Hidemitsu

AU - Toyono, Takashi

AU - Toyoshima, Kuniaki

AU - Ohuchi, Hideyo

PY - 2002

Y1 - 2002

N2 - Mouse incisors have a cervical loop that gives rise to dental epithelium in the apical region of the tooth germ, in contrast to molars. In a study of formation of the stem-cell compartment, we focused on expression patterns of fibroblast growth factor (Fgf) 10 and Fgf3 in developing mice incisors. At E14, Fgf10 and Fgf3 were coexpressed in the dental papilla. After E16 mesenchymal cells underlying cervical loop expressed Fgf10 but not Fgf3. To illustrate the role of FGF10, we analyzed incisor development of Fgf10-deficient mice. The germs of FGF10-null mice proceeded to cap stage normally. However, at a later stage, the cervical loop was not formed. Functional disorder of FGF10 by a neutralizing anti-FGF10 antibody induced apoptosis in the cervical loop of incisor explants. Recombinant FGF10 rescued the cervical loop from apoptosis. These results show that FGF10 maintains the stem-cell compartment in the developing incisor tooth germ.

AB - Mouse incisors have a cervical loop that gives rise to dental epithelium in the apical region of the tooth germ, in contrast to molars. In a study of formation of the stem-cell compartment, we focused on expression patterns of fibroblast growth factor (Fgf) 10 and Fgf3 in developing mice incisors. At E14, Fgf10 and Fgf3 were coexpressed in the dental papilla. After E16 mesenchymal cells underlying cervical loop expressed Fgf10 but not Fgf3. To illustrate the role of FGF10, we analyzed incisor development of Fgf10-deficient mice. The germs of FGF10-null mice proceeded to cap stage normally. However, at a later stage, the cervical loop was not formed. Functional disorder of FGF10 by a neutralizing anti-FGF10 antibody induced apoptosis in the cervical loop of incisor explants. Recombinant FGF10 rescued the cervical loop from apoptosis. These results show that FGF10 maintains the stem-cell compartment in the developing incisor tooth germ.

KW - Epithelial-mesenchymal interactions

KW - FGF10

KW - Incisor development

KW - Stem cells

UR - http://www.scopus.com/inward/record.url?scp=0035996478&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035996478&partnerID=8YFLogxK

M3 - Article

C2 - 12489159

AN - SCOPUS:0035996478

VL - 43

SP - 201

EP - 204

JO - Connective Tissue Research

JF - Connective Tissue Research

SN - 0300-8207

IS - 2-3

ER -