Fetuin mediates hepatic uptake of negatively charged nanoparticles via scavenger receptor

Susumu Nagayama, Ken ichi Ogawara, Keiko Minato, Yoshiko Fukuoka, Yoshinobu Takakura, Mitsuru Hashida, Kazutaka Higaki, Toshikiro Kimura

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

We tried to evaluate the possible involvement of fetuin in the scavenger receptors (SRs)-mediated hepatic uptake of polystyrene nanospheres with the size of 50 nm (NS-50), which has surface negative charge (zeta potential = -21.8 ± 2.3 mV). The liver perfusion studies in rats revealed that the hepatic uptake of NS-50 pre-coated with fetuin (NS-50-fetuin) was significantly inhibited by poly inosinic acid (poly I), a typical inhibitor of SRs, whereas that of plain NS-50 or NS-50 pre-coated with BSA (NS-50-BSA) was not. The uptake of NS-50-fetuin by cultured Kupffer cells was also significantly inhibited by poly I, and anti-class A scavenger receptors (SR-A) antibody, suggesting that fetuin on NS-50 mediated the recognition and internalization of NS-50 by Kupffer cells and at least SR-A would be responsible for the uptake. Taken that Western blot analysis confirmed that fetuin certainly adsorbed on the surface of NS-50 after the incubation of NS-50 with serum, the results obtained in the present study indicate that fetuin would be one of the serum proteins that were substantially involved in the hepatic uptake of NS-50 via SRs.

Original languageEnglish
Pages (from-to)192-198
Number of pages7
JournalInternational Journal of Pharmaceutics
Volume329
Issue number1-2
DOIs
Publication statusPublished - Feb 1 2007

Fingerprint

Fetuins
Scavenger Receptors
Nanoparticles
Liver
Poly I
Kupffer Cells
Class A Scavenger Receptors
Nanospheres
Inosine Monophosphate
Polystyrenes
Blood Proteins
Cultured Cells
Perfusion
Western Blotting
Antibodies
Serum

Keywords

  • Fetuin
  • Hepatic uptake
  • Polystyrene nanosphere
  • Receptor-mediated phagocytosis
  • Scavenger receptors

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Nagayama, S., Ogawara, K. I., Minato, K., Fukuoka, Y., Takakura, Y., Hashida, M., ... Kimura, T. (2007). Fetuin mediates hepatic uptake of negatively charged nanoparticles via scavenger receptor. International Journal of Pharmaceutics, 329(1-2), 192-198. https://doi.org/10.1016/j.ijpharm.2006.08.025

Fetuin mediates hepatic uptake of negatively charged nanoparticles via scavenger receptor. / Nagayama, Susumu; Ogawara, Ken ichi; Minato, Keiko; Fukuoka, Yoshiko; Takakura, Yoshinobu; Hashida, Mitsuru; Higaki, Kazutaka; Kimura, Toshikiro.

In: International Journal of Pharmaceutics, Vol. 329, No. 1-2, 01.02.2007, p. 192-198.

Research output: Contribution to journalArticle

Nagayama, S, Ogawara, KI, Minato, K, Fukuoka, Y, Takakura, Y, Hashida, M, Higaki, K & Kimura, T 2007, 'Fetuin mediates hepatic uptake of negatively charged nanoparticles via scavenger receptor', International Journal of Pharmaceutics, vol. 329, no. 1-2, pp. 192-198. https://doi.org/10.1016/j.ijpharm.2006.08.025
Nagayama, Susumu ; Ogawara, Ken ichi ; Minato, Keiko ; Fukuoka, Yoshiko ; Takakura, Yoshinobu ; Hashida, Mitsuru ; Higaki, Kazutaka ; Kimura, Toshikiro. / Fetuin mediates hepatic uptake of negatively charged nanoparticles via scavenger receptor. In: International Journal of Pharmaceutics. 2007 ; Vol. 329, No. 1-2. pp. 192-198.
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